PrimeTime & Surveyor Mutation Assays by IDT

PrimeTime qPCR Assays

PrimeTime qPCR assays come in two formats: probe based assays and primer only assays.

Probe based assays may be ordered with a variety of dye and quencher combinations, in three different scales: 100, 500 or 2500 reactions. Predesigned human, mouse and rat assays are available, and IDT also provides tools to help with the design of custom assays.

Primer only assays are ideal for using with intercalating dye experiments. Each order of these primers is enough for 500 reactions.

Molecular Beacon assays (highly specific, dual labeled, hairpin based probes) are also available with a variety of dye/quencher combinations.


Surveyor Mutation Assays

Designed to detect mutations in DNA derived from a variety of organisms including bacteria, fungi, plants, and animals, Surveyor® Mutation Detection Kits allow analysis by:

Standard gel electrophoresisWAVE® and WAVE HS Systems

Surveyor Nuclease is provided as a standardized, quality-controlled enzyme formulation in a ready-to-use kit that can be used without further batch-to-batch optimization. All components except the thermostable DNA polymerase and PCR products are provided. Reaction buffers and positive controls included in the kit allow users to set up and monitor the assays to obtain reproducible results. In-depth Surveyor Mutation Detection Kit User Guides provide detailed instructions and troubleshooting.

Two types of Surveyor Mutation Detection Kits are available:

Surveyor Mutation Detection Kit for Standard Gel Electrophoresis—S25 (25 rxn), S100 (100 rxn), S1000 (1000 rxn)Surveyor Mutation Detection Kit for WAVE® and WAVE HS Systems—W25 (25 rxn), W100 (100 rxn), W1000 (1000 rxn)

Pricing

Probe based AssaysPrice
Mini (100 rxns)$80.00
Standard (500 rxns)$108.00
XL (2500 rxns)$315.00

Taqman Assays by Thermofisher

ThermoFisher Scientific custom products are available through the GRCF. By ordering through us, you will receive small discount and free shipping on all orders! Orders can be delivered to you lab via the Core Store delivery mechanism or picked up in our office in Blalock 1004. Products available include:

Taqman Gene Expression Assays, Plates and ArraysTaqMan MicroRNA AssaysSNP Genotyping, CNV, siRNA, and mutation detection assayssiRNAs

Pricing

Pricing for some commonly ordered items is listed below. This is far from an exhaustive list. If you can find it using the tool above, you can order it through our facility.

Part NumberDescriptionPrice
4448892 & 4453320Gene Expression assays, inventoried or made to order, Fam label, size XS$68.35
4331182Off the shelf Gene Expression assays, Fam label, size small$151.72
4448484Gene Expression assays, made to order, size small, VIC-MGB-PL$241.72
4448489Gene Expression assays, made to order, size small, VIC$263.88
4351372Gene Expression assays, made to order, size small, FAM$256.00
4351370Gene Expression assays, made to order, size medium, FAM$404.37
4351368Gene Expression assays, made to order, size large$867.67
4351379Taqman SNP assays, human, size small$288.74
4427975Taqman MicroRNA assays, size small$214.57
4390825Sil. Sel. Validate siRNA, STD. 20NM$493.53
4464066mirVana Mimics & Inhibitors, S$285.40

Ordering Tool

To place your order, use the tool from ThermoFisher below. Once your cart is full, submit the order, providing the requested information. The tool will email your order to our facility. We will place the order, email you a confirmation and deliver the assays when they arrive. The tool will email us a shopping cart and we will place your order.  You will receive an email when the order has been placed.

Ordering Instructions

Custom Taqman assays may be ordered by following these steps:

  1. Create your custom design using the  gene expression or  SNP design tools on the ThermoFisher website.
  2. Place the assays you wish to order in a shopping cart.
  3. Transfer control of the shopping cart to customorders@jhmi.edu
  4. Provide us with the name of your PI, your PI’s email address, the budget number and building and room number for delivery.
  5. We will place the order and send you an email verification.


If you are unsure of how to select the best microRNA assays for your experiment, check out the  FAQ notes developed by ThermoFisher.


Guidelines/Policies

Orders

All orders are final.

Orders cannot be altered once placed. If you need to change something about your order, please call us immediately upon emailing the shopping cart. Once you receive the email stating that your order has been placed, it is too late to change it.

If you have questions regarding your order, please  contact us or call 410-955-2836.

Payment

You must provide a Johns Hopkins budget number or a KKI PO when placing oligo orders. Do not provide a credit card number or a PO number from SAP. We cannot use them as payment.

Delivery

Taqman products may take 2-7 days for delivery. You will be provided an estimated shipping date upon placing your order. If your order is delayed beyond that, please  contact us or call 410-614-0702.

If no one is present in your lab when your order is delivered, it will be returned to our facility. We will not leave an order without a signature.

If you wish to pick up your order, but selected delivery when you placed your order, please call 410-955-2836 the morning you are expecting your order and we will set them aside for pick up. Please have your order number ready when calling.

Shipping charges

There are no shipping charges for any Taqman orders placed through our portals.


Frequently asked questions

How do I set up a GRCF portal account with my favorite company? Is each company a different login?
  • For Dharmacon, if you have a current account, you do not need to create a new account. Simply starting on  our portal login page will associate your account with our portal. If you need to create an account, you can do so on the  registration pageOrders created on the Dharmacon portal create a quote which is emailed to the GRCF. The GRCF will place the orders by the end of the day.  You will receive an email when the order has been placed.


  • For IDT, you must create a  portal specific account, but account set-up is instantaneous. Simply go to our portal and create a new account. Make sure you do not click anywhere else on the page prior to creating the account, or the web-site will lose the association with our portal. Once you have set up the account, you may place your order. When placing your first order on a newly created account, you will see incorrect pricing and shipping charges, but your bill will be correct.


  • For MilliporeSigma, you need to email us the following information:
  • Your name
  • Your email address
  • Your phone number
  • Your building and room number (for CoreStore deliveries)
  • Your PI’s name & email address
  • If you already have set up a GRCF account with MilliporeSigma, you may order using  this link.


  • ThermoFisher and Qiagen do not require an account. Shopping carts are emailed to the GRCF (customorders@jhmi.edu) for ordering.
How long does it take to activate my account?

Dharmacon and IDT are instantaneous. If you already have a Dharmacon direct account, you may simply login on our  portal page and your orders will be associated with the portal. If you already have a direct IDT account, you MUST create a new account by clicking “Set up new account” on our  portal page (without clicking anywhere else on the page.)

For MilliporeSigma, you must create an account and wait for an email indicating that the account is active. This may take up to two business days.

ThermoFisher and Qiagen do not require an account. Orders are emailed to us and placed by the GRCF staff.

I already have an account set up with this company on their standard web-site. May I use that account?

No. You must create an account specific to the GRCF portal in order to take advantage of our pricing. The exception to this is Dharmacon. Prior Dharmacon accounts work, as long as you start on our  portal page.

Do I need a purchase order to place an order? Can I pay with an IO number?

On any of these sites, you will pay with your budget number. You do not need a credit card or a PO number from SAP. KKI customers should enter the last name of the PI on their grant or a KKI PO number. All customers (KKI and JHU) will be billed through iLab. JHU customers should check to make sure their budget numbers are active in iLab. Non-sponsored budget numbers must be added to iLab manually by following  these instructions.

When will I receive my items?

Standard oligo orders placed before noon from MilliporeSigma arrive the next business day. Standard oligo orders placed with IDT before 2 PM will arrive in our office two business days after they are placed.  Other items vary depending on the time it takes the manufacturer to ship.  All items are available in Blalock 1004 the day they are received and will go out for delivery with the CoreStore the next morning.

Will you leave the order in my lab if no one is there?

No. Due to the sensitive nature of some of the items ordered through our portals, someone must be present in your lab for your order to be delivered.

May I pick up my order, even if I indicated I wanted delivery when I placed the order?

Yes. On a typical day, we receive the orders by 11:30 AM. Orders are available in Blalock 1004 from the time they arrive until we close at 4:30 PM. You may stop by and pick them up anytime during those orders. If you call ahead, please be prepared to tell us the order number and then name of the person placing the order. Orders are brought to the CoreStore early the following morning and set out for delivery.

If you know that you would like to pick up your order at the time you place your order, you may replace your building and room number with the words “Pick up” and we will contact you when the order arrives.  Pickup orders are placed in one of our lockers near the Blalock elevators and are available 24/7.

I order for more than one lab. How do I change where an order is being delivered?

You should create a separate ordering account for each lab to make sure that your orders are delivered to the correct location.

How do I order items that require a special quote?

To order something that requires a special quote, please   email us all of the necessary information and we will reach out to your chosen company and generate the quote. This will ensure that our special pricing and free shipping are applied.

Why do I see shipping charges when placing an order from IDT?

This usually means that you have logged into the portal using a username and password that were set up for the SAP punchout or the normal IDT web-site. Using the wrong login will take you to the direct website without any notification. You will only receive free shipping if you use a username and password set up specifically on our portal. You will know you are on the correct website if you see a banner that says “You are using the DNA Analysis Facility Portal” on every page. Also, when you begin the checkout process, the billing and shipping information should be prepopulated with the GRCF address and PO. Click past this page and you will come to a page that asks for your budget number and building and room number. If your account is set up properly and you see shipping charges, you have probably chosen the “expedite” products. See the image below for the correct selection for avoiding those expedite fees.


Location

Johns Hopkins East Baltimore Medical Campus

600 N. Wolfe Street

Blalock Building, Room 1004

Baltimore, MD 21287 USA

Hours

Monday – Friday:

7:00 am to 4:30 pm

After-hours pickup is available from the lockers near the Blalock elevators. Please contact us to make arrangements.

Contact

Phone

410-955-2836