AT THE GRCF HIGH THROUGHPUT SEQUENCING CENTER, OUR GOAL IS TO PROVIDE THE RESEARCH COMMUNITY AT JOHNS HOPKINS UNIVERSITY WITH ACCESS TO ‘NEXT GENERATION’ SEQUENCING PLATFORMS.
We currently feature MiSeq sequencing.
We do our best to sequence samples in a timely manner, but our first priority is good data.
We offer simple, per lane pricing, along with end to end service for whole exome/custom targeted projects, RNASeq, and whole genome.
MiSeq reagents are sold by cycle number. They can be run in single/paired end, and/or indexed mode.
50 cycles (v2):
$1000 per run
150 cycles (v3):
$1,100 per run
300 cycles (v2):
$1,200 per run
500 cycles (v2):
$1,300 per run
600 cycles (v3):
$1,500 per run
Data Yield is dependent upon several factors:
The longer the read, the more data.
Paired end reads yield twice the data as single read.
Optimal Cluster Density
It is imperative to accurately quantitate your library to ensure high data yield. We do our best to QC libraries before sequencing, but we cannot pool samples for you.
High Quality Library
Libraries that contain a high level of adapter dimers will yield significantly less data, as the fragments will hybridize to the flowcell. Similarly, over amplified libraries can negatively impact yield.
Uniform Base Composition
Libraries that have uneven base composition tend to pose problems with the MiSeq analysis software. These issues can be mitigated using several strategies, but the net effect will be lower data yield per lane than a balanced library.
Data will be returned in Sanger FASTQ format via our high speed aspera server.