Taqman Gene Expression Arrays may be ordered and run through the GRCF
Applied Biosystems by ThermoFisher’s OpenArray chips are ideal for investigators who wish to look at multiple assays on medium to large numbers of samples. They come in fixed and custom content options.
Understanding the OpenArray
The technology spots Taqman assays onto chips, which are then run in our QuantStudio 12K flex machine.
The number of samples on each chip varies, based on fixed content or custom option chosen.
Custom chips can be formatted with 18, 56, 112, 168 or 224 assays.
There is a minimum order of 10 custom chips, but smaller projects are often cost effective, even when excess chips must be ordered.
Fixed Content Panels
Human Cancer Panel: This panel targets genes related to DNA repair, angiogenesis, cell adhesion, and ECM, as well as genes involved in the cell cycle and apoptosis, and many of the genes encoding kinases and transcription factors that have been found to be differentially expressed in early cancer and metastatic disease. The 624 genes have been validated for pluripotency. Twenty-four endogenous controls are included for normalization. Each chip tests 4 samples.
Human Inflammation Panel: This panel covers 586 genes that have been studied as targets for a range of inflammatory disease, plus 21 endogenous control genes. Each chip tests 4 samples.
Mouse Inflammation Panel: This panel covers 632 genes that have been studied as targets for a range of inflammatory diseases, plus 16 endogenous control genes. Each chip tests 4 samples.
Human Kinome Panel: This panel targets 772 well defined genes for human kinases and provides 56 endogenous control genes for normalization. Genes for the panel were chosen based on primary literature and publications. Each chip tests 3 samples (or one sample in triplicate).
Human Signal Transduction Panel: This panel contains 573 signal transduction-related genes plus 24 endogenous controls for normalization. The JAK-STAT, NFκB, Akt, GPCR, cAMP, and MAP kinase pathways are well represented. Each chip tests 4 samples.
Stem Cell Panel: This panel targets 609 genes validated for pluripotency, plus 22 endogenous controls for normalization. They use a well-defined set of validated gene expression markers to characterize human embryonic stem (ES) cell identity and assess phenotypic variations between embryonic stem cell isolates. The gene content of this OpenArray® plate resulted directly from the work of the I.S.C.I consortium and characterization of human embryonic stem cell lines by the International Stem Cell Initiative. Each chip tests 4 samples.
Pricing:
1 chip*: $1,300
2 chips: $2,100
3 chips: $2,900
4 chips: $3,700
Discounts available for projects of 11 or more of the same chip. Please inquire.
*Sample number varies by pathway. See descriptions above for specifics. Human Signal Transduction Panel, add $200 per chip.
Custom Array Pricing
Pricing varies by number of gene expression assays and sample numbers. Chips which use custom design assays are more expensive then chips that use only inventoried assays.
Examples using minimum numbers, for both options, are given below, but please contact us for detailed information.
Please note that if you would run your samples in triplicate in a standard gene expression assay, you should do so with these assays as well. Sample numbers given below do not take that into consideration.
| Chip Format | # Samples/Chip | Total Samples | Price | Total cost** |
|---|---|---|---|---|
| 18 Assays* | 48 | 480* | Chips: $15,380 Other costs: $1950 | $17,330 |
| 56 Assays | 48 | 480 | Chips: $17,205 Other Costs: $1950 | $19,155 |
| 112 Assays | 24 | 240 | Chips: $18,270 Other costs: $3,900 | $22,170 |
| 168 Assays | 16 | 160 | Chips: $20,260 Other costs $5,850 | $26,110 |
| 224 Assay | 12 | 120 | Chips: $21,045 Other costs: $7,800 | $28,845 |
*18 assay formatted shapes are plated in triplicate, so each sample is used once, but run 3 times. The formats are single sample numbers, not run in triplicate.
How do I submit my samples?
Customers should prepare cDNA from total RNA, following recommended procedures, and fill out our online request form. Prepared cDNA should be brought to Blalock 1004 or dropped off in one of our remote locations in the appropriate number of 96 well plates. Mark each plate with the order number from the online request. Data will be returned to you via our ordering server.
Location
Johns Hopkins East Baltimore Medical Campus
600 N. Wolfe Street
Blalock Building, Room 1004
Baltimore, MD 21287 USA
Hours
Monday – Friday:
7:00 am to 4:30 pm
After-hours pickup is available from the lockers near the Blalock elevators. Please contact us to make arrangements.
