Convenient, on-site sample processing for your clinical or research study
The GRCF Cell Center is a CAP-accredited facility specializing in blood isolations, mammalian cell culture propagation, primary cell establishment, and EBV-transformed lymphoblast (LCL) establishment. Our facility has processed more than 40,000 blood specimens and serves the needs of hundreds of investigators within Johns Hopkins and beyond.
Services offered include isolation and cryopreservation of whole blood, lymphocytes, sera, and plasma. Lymphoblast establishment (LCL) service is also available.
Cell Culture Services
Services offered include primary fibroblast establishment, cell culture expansion, and cell line distribution. The GRCF Cell Center is a mycoplasma-free facility.
Clinical Trial Support
Services offered include processing, storage, and shipment of blood isolates, urine, saliva, nasal secretions, stool sampling, breast milk, and more.
Services offered include processing, storage, and shipment of COVID-19 biospecimens.
Johns Hopkins East Baltimore Medical Campus
600 N. Wolfe Street
Blalock Building, Room 1001A
Baltimore, MD 21287 USA
Monday – Thursday:
8:30 am to 6:00 pm
Friday: 8:30 am to 5:30 pm
Same Day Processing Cutoff:
Monday – Thursday: 3:30pm
All clinical samples needing same day processing after these hours will be subject to additional fees.
How to Setup Processing
The GRCF Cell Center requests an initial consultation to discuss your project needs prior to dropping off your first samples. Please contact us at 410-955-3320 or by email at email@example.com to setup an account for processing in our facility.
The following documentation must be completed and submitted prior to dropping off first samples:
1) Completed Project Information Form
3) IRB Approval Letter
4) Blank copy of the Informed Consent
5) Letter from Principal Investigator affirming all patients have consented to the study
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Viable nucleated cells are cells that are “able to live”. Nonviable cells are incapable of further growth. Snap-frozen cells are not capable of further growth because the cell membrane is shattered by intracellular ice. However, when frozen at -1C per minute with a protectant such as dimethylsulfoxide (DMSO), cells are “suspended in animation”. The cryoprotectant allows intracellular water to be removed slowly, ensuring that organelles are protected from ice formation. To preserve viability, cells are thawed rapidly at 37C and placed into growing media.
Cells that have been properly frozen can remain in liquid nitrogen vapor for decades without loss of viability. The Cell Center has revived samples that have been stored for 17 years with a post-freeze viability > 90%.
The lab has separated over 24,000 blood samples.
The success rate for transforming fresh lymphocytes is 99% and for frozen lymphocytes, 98%.
Over 400 fibroblast lines have been established at The Cell Center.
The blood should be inverted several times to mix the preservative and blood.
One day. After that, the likelihood of a good separation decreases. After 3 days, the viability is low.
No, keep the blood tubes at room temperature, protecting from extremes of hot and cold during transport.
The ACD (acid citrate dextrose) yellow-top tube.
The ACD (acid citrate dextrose) in solution A is concentrated for 8.5 mls of blood. The ACD in solution B is concentrated for 6 mls of blood.
Yes. From our experience, if an 8.5 ml tube is not filled, the cells will rupture because of the concentrated preservative. Use the smaller (6 ml) tube if there is a chance of low volume.
Blood samples must arrive at the lab by no later than 2 pm.
Yes. If blood for lymphocyte isolation is not collected in an ACD tube, if the tube is not full and the volume of blood is not correct for the preservative concentration, or if the time from collection is greater than 3 days the sample will not pass our quality control at intake.
No, but another lab within the Genetics Resources Core Facility does. Contact the manager of the GRCF Fragment Analysis Facility Lab at 410-614-3830.