GRCF Cell Center Services
The GRCF Cell Center offers same day service aliquoting and/or cryopreservation of various blood isolates including whole blood, lymphocytes, sera, plasma, and more. Our facility also specializes in the establishment of cell lines (LCLs) from isolated lymphocytes or whole blood.
Viable Lymphocytes from Whole Blood
Sterile lymphocyte isolation by Ficoll gradient, cell count, viability determination, cryopreservation of 1 mL aliquot whole blood, cryopreservation of 1-4 aliquots of isolated lymphocytes at 5e6 cells/vial.
Sera and Plasma
Isolation and cryopreservation of sera and plasma aliquots (0.5 – 1mL). Cryopreserved proteins, antibodies, antigens, enzymes, hormones, and metabolites are intact for future analysis.
Cell Lines (LCLs) from Isolated Lymphocytes
B-cell transformation from whole blood isolated blood lymphocytes, culture expansion, and cryopreservation. Cryopreservation of whole blood aliquot (1 mL), 1-4 aliquots of lymphocytes at 5e6 cells, and 4 aliquots of (transformed) lymphoblasts at 5e6 cells. Please allow 8-10 weeks for completion.
Whole Blood
Aliquoting fresh whole blood in 0.5 – 1mL volumes and cryopreserving for DNA or other research purposes.
Starting Up
How do I start using the GRCF Blood Processing service?
The GRCF Cell Center requests an initial consultation to discuss your project needs prior to dropping off your first samples. Please contact us at 410-955-3320 or by email at [email protected] to setup an account for processing in our facility.
The following documentation must be completed and submitted prior to dropping off first samples:
- Completed Project Information Form
- Signed GRCF Biorepository & Cell Center Agreement Form
- IRB Approval Letter
- Blank copy of the Informed Consent
- Letter from Principal Investigator affirming all patients have consented to the study
After-Hours Drop Off
For your convenience, the GRCF Cell Center has an after hours drop off system for blood processing orders! Blood can be deposited in secure After Hours Drop Boxes or our 4 deg C fridge for processing at 8:30 am the following business day.
PLEASE NOTE: This system is only available to customers previously setup for blood processing. Please ask us about how to use this system for your future drop offs.
