Taqman MicroRNA Profiling

The GRCF is proud feature Taqman MicroRNA profiling using the QuantStudio 12K Flex Real-Time PCR System. This new, high throughput technology allows us to analyze dozens of samples in a day, generating thousands of data points, at reasonable prices, using highly sensitive and highly specific Taqman assays.

Quant Studio 12K

The QuantStudio 12K allows you to look up to 758 unique assays per sample, quickly and efficiently with only 200ng of total RNA needed for a full miRNA profile. We can generate the microRNA specific cDNAs from your total RNA sample for an additional charge.

How to request MicroRNA Profiling

#1 Sample Prep

Customers will need to prepare the cDNA according to Life Technologies  quick reference guide, or utilize our  cDNA synthesis service.

Taqman MicroRNA Reverse Transcriptase kits can be purchased from the GRCF Core Store in  200 rxn or  1000 rxn sizes. Megaplex primers should be picked up from the GRCF DNA services lab in Blalock 1004, or we can arrange for them to be delivered to your lab. When the first stopping point is reached, aliquot 5 ul of each template/primer pool combination into 12-strip PCR tubes. (Each original template should have cDNA made from pool A and pool B, so each original template should have 2 final tubes. 


#2 Drop Off


Fill out our  online request form and bring samples to Blalock 1004 or one of our  remote drop off locations, labeled with the order number from the online request.

Once we receive your cDNA, we will complete the rest of the protocol and generate the data. Data will be returned to you via our ordering server.

Please note: If you are bringing more than 12 samples, do not put into a plate or we will be forced to freeze/thaw your samples multiple times).


Pricing

Number of SamplesStandard miRNA AssaysAdvanced miRNA Assays
3$950$840
6$1,600$1400
9$2,200$1960
12$2,800$2520

There are additional price breaks at 30 samples and at 300 samples. If you would like pricing for larger numbers of samples or if you wish to do the preamplification yourself, contact the GRCF at 410-955-2836 or   customorders@jhmi.edu. RNA extraction and cDNA synthesis services are also available. Please inquire. *Samples must be run in multiples of 3 (or in triplicate).