SP: ~800 million paired reads, ~400 million single reads

 SP: ~1.6 billion paired reads, S1: ~3.2 billion paired reads, S2: ~8.2 billion paired reads, S4: ~20 billion paired reads

We no longer offer HiSeq2500 runs due to cost. Please consider ordering a single lane on NovaSeq. If you have a legacy project that requires HiSeq, please  contact David Mohr

GRCF High Throughput Sequencing Center

Our goal is to provide the Johns Hopkins community access to Next Generation sequencing platforms.

We currently feature  MiSeq and  NovaSeq6000 instruments. We do our best to sequence samples in a timely manner, but our primary focus is on generating high quality data.

Pricing Tables

NovaSeq runs include additional cycles for dual indexed libraries.

If you need to individually load lanes on the NovaSeq, it adds $150/lane

SP: ~1.6 billion paired reads, ~800 million single readsS1: ~3.2 billion paired reads, ~1.6 billion single readsS2: ~8.2 billion paired reads, ~4.1 billion single readsS4: ~20 billion paired reads, ~10 billion single reads
100 cycle: $3600 100 cycle: $6000 100 cycle: $9300
200 cycle: $4300200 cycle: $7000200 cycle: $11100200 cycle: $16000
300 cycle: $4600300 cycle: $7500300 cycle: $11600300 cycle: $17400
500 cycle: $5800

NovaSeq Per Lane Pricing

SP: ~800 million paired reads, ~400 million single reads
100 cycle: $1950
200 cycle: $2300
300 cycle: $2450
500 cycle: $3050

High Throughput Sequencing gives you options… 

We offer free consultation. Please  contact David Mohr to discuss your project in detail.

We offer simple, per lane and per flow cell pricing, along with end to end service for whole exome/custom targeted projects, RNASeq, and whole genome.

For NovaSeq 6000 lane loading, we now offer single lane loading on NovaSeq SP to accommodate users who do not need the throughput of a full flowcell.

Sample Requirements

NovaSeq Runs

For NovaSeq runs, please submit at least 50ul of your sample at 4nM.


Samples should be pooled. 

QC Preferences

Nanodrop is not  reliable. Intercalating dye methods or qPCR are preferred.

MiSeq Runs

Please submit 10μl of your sample at 2nM for MiSeq. 

Quality Control

We will do a QC check via Bioanalyzer to increase the likelihood of quality data, but you should quantitate your sample as accurately as possible prior to submission. 


Please contact us with any sample preparation questions.

DNA or total RNA

 Please provide us with ~500ng of high molecular weight DNA or total RNA >6.5.

Low yield

There are a host of options for lower input/lower quality that may add cost.




Please  contact us to discuss sample submission or any questions you may have regarding library preparation.

Understanding Data Yield

Our facility features the NovaSeq platform. Yield is dependent upon several factors:

Read Length & Read Type

The longer the read, the more data. Paired end reads yield twice the data as single read.

Uniform Base Composition

Libraries that have uneven base composition tend to pose problems with the analysis software. These issues can be mitigated using several strategies, but the net effect will be lower data yield than a balanced library.

Optimal Cluster Density

 It is imperative to accurately quantitate your library to ensure high data yield. We do our best to QC libraries before sequencing, but we cannot pool samples for you.

High Quality Library

Libraries that contain a high level of adapter dimers will yield significantly less data, particularly on the NovaSeq6000. Similarly, over amplified libraries can negatively impact yield

Please see Illumina’s  NovaSeq6000 Specification page for current data yields. While we regularly achieve greater than ‘spec’ yields from the NovaSeq6000, it is best to be conservative when planning your experiments.

Data Delivery

Per lane/flowcell sequencing

Data will be returned in Sanger FASTQ format via our high speed aspera server.

End to end services

Alignment files, variant calls, and any intermediate files you wish via aspera server.