cDNA Synthesis for MicroRNA Profiling and Custom Gene Expression Taqman Arrays
The GRCF offers cDNA synthesis to support customers requesting:
Taqman Open Array
MicroRNA Profiling Analysis
CustomFixed Gene Expression Array analysis on the QuantStudio 12K Flex Real-Time PCR System
cDNA synthesis for Gene Expression Array analysis
Superscript VILO, Life Technologies part # 4453650
cDNA synthesis for MicroRNA profiling
inquire for A and B pools
High Capacity cDNA Reverse Transcription Kit, Life Technologies part # 4368813
The recommended amount of total RNA for preparing cDNA for microRNA Profiling is 100ng.
The recommended amount of total RNA for preparing cDNA for Gene Expression Taqman Arrays is 10µl at 200ng/µl (2µg).
Qualitative analysis of RNA samples is available by Advanced Analytical Fragment Analyzer CE analysis. The system determines a RQN (RNA Quality Number) of total RNA on a scale from 1 to 10 that correlates to current industry practices (RIN).
cDNA Synthesis Questions: Please contact Alejandra Gutierrez (email@example.com)
Please contact Roxann Ashworth (firstname.lastname@example.org) for all other questions including: Taqman OpenArray, MicroRNA Profiling Analysis or Gene Expression Array analysis on the QuantStudio 12K Flex Real-Time PCR System