cDNA Synthesis for MicroRNA Profiling & Gene Expression Taqman Arrays

cDNA Synthesis for MicroRNA Profiling and Custom Gene Expression Taqman Arrays

The GRCF offers cDNA synthesis to support customers requesting:

 Taqman Open Array
MicroRNA Profiling Analysis
CustomFixed Gene Expression Array analysis on the QuantStudio 12K Flex Real-Time PCR System

Pricing

cDNA synthesis for Gene Expression Array analysis

$ 26
per sample

Superscript VILO, Life Technologies part # 4453650

cDNA synthesis for MicroRNA profiling

inquire for A and B pools

 High Capacity cDNA Reverse Transcription Kit, Life Technologies part # 4368813

How to Order

Ordering

Please contact  Dr. Gutierrez for cDNA synthesis ordering.

Please contact  Roxann Ashworth [410-614-0702] for Taqman OpenArray, MicroRNA Profiling Analysis, or custom and fixed content Gene Expression Array analysis on the QuantStudio 12K Flex Real-Time PCR System .

RNA Requirements

  • RNA should be free of PCR inhibitors
  • Has a A260/230 ratio between 2.0 and 2.4
  • Has a A260/280 ratio between 1.8 and 2.1
  • Has a RNA Integrity Number (RIN) or RNA Quality Number (RQN) between 6.5 and 10

The recommended amount of total RNA for preparing cDNA for microRNA Profiling is 100ng.

The recommended amount of total RNA for preparing cDNA for Gene Expression Taqman Arrays is 10µl at 200ng/µl (2µg).

Qualitative analysis of RNA samples is available by  Advanced Analytical Fragment Analyzer CE analysis. The system determines a RQN (RNA Quality Number) of total RNA on a scale from 1 to 10 that correlates to current industry practices (RIN).

Questions

  cDNA Synthesis Questions: Please contact Alejandra Gutierrez (mgutie25@jhmi.edu)  

Please contact Roxann Ashworth (rashwor2@jhmi.edu) for all other questions including:  Taqman OpenArray, MicroRNA Profiling Analysis or Gene Expression Array analysis on the QuantStudio 12K Flex Real-Time PCR System