The GRCF is excited to offer genomic applications for single cells

including RNA-seq, gene expression profiling by qPCR and DNA amplification for whole-genome or targeted (exome or PCR-based analysis) through  10x Genomics Chromium platform.

An Overview of the Technology

High-throughput molecular barcoding

Cell-by-Cell 3’ end counting of mRNA transcripts

Tens of thousands of cells per run

Training for cellular capture with the GRCF

10x Genomics Chromium System

 This technology supports a broad range of applications, including cancer-cell transcriptomics and cell-type identification and discovery. Because the platform works with short read sequencers, it integrates easily into the existing GRCF RNAseq workflow.

Reserve Time on the Chromium System

The GRCF is currently offering ‘self-service’ and training opportunities on the 10x Genomics Chromium system. The well-characterized pipeline and unique software, allows the GRCF to offer analysis for 10X Genomics data. The GRCF  cost for sequencing will vary based on read length.

Single Cell Genomics through the GRCF is a joint effort between the Cell Center & DNA Services

We offer support on single cell isolation, training for capture and sequencing and analysis service.

Contact us today for more information or to schedule for your experiment.

bioprocessing@jhmi.edu