Featuring PrimeTime gene Expression assays and the rhAmp Genotyping system
Featuring Quantinova probe and PCR assays, dPCR assays and SNP detection assays
Featuring Taqman Assays for Gene Expression, SnP and Copy number Variation
dPCR/qPCR Assays
Differences and changes in gene expression are important measures to understand biological systems. One of the most widely used methods of analyzing gene expression is quantitative PCR (qPCR) following cDNA synthesis. qPCR, also called real-time PCR, uses a fluorescent reporter molecule with a primer pair to measure target nucleic acid amplification during PCR cycling. Most qPCR assays can also be used for dPCR
Our partner companies offer a variety of tools for performing qPCR, including off the shelf assays (both probe based and intercalating dye based) and custom assays.
PrimeTime Assays from IDT
PrimeTime qPCR assays come in two formats: probe based assays and primer only assays.
Probe based assays may be ordered with a variety of dye and quencher combinations, in three different scales: 100, 500 or 2500 reactions. Predesigned human, mouse and rat assays are available, and IDT also provides tools to help with the design of custom assays.
Primer only assays are ideal for using with intercalating dye experiments. Each order of these primers is enough for 500 reactions.
Primer and probe sequences are provided with all orders.
QuantiNova Assays from Qiagen
QuantiNova LNA assays come in two formats: probe based assays and primer only assays.
Choose from a wide selection of predesigned assays for accurate and sensitive detection of any human, mouse or rat mRNA or lncRNA – from general transcript detection to differentiation of specific transcript isoforms. LNA enhancement allows the design of shorter primers and probes, which are more flexible to position on the target, providing opportunities to design for specific transcript detection or target differentiation. Plus, thorough design validation ensures optimal performance and robust detection, and assays for all popular mRNAs and lncRNAs undergo wet-lab validation.
Taqman Assays from ThermoFisher
TaqMan Gene Expression assays are built on more than 20 years of real-time PCR gene expression experience. Each assay includes target primers and a sequence-specific probe optimized for the best functional performance. No additional design, optimization, or melt curve analysis is needed. Available in a wide variety of formats and species, new assay designs are constantly added to help meet your research needs. TaqMan assays have been cited in over 40,000 publications and are backed by more than 350 patents.
A few things to know
Specialized quotes from must be handled outside of the portal through contact with the local sales representative and the GRCF. Please email us with the details of what you would like to order.
IDT ACCOUNt Creation
You can create an account on IDT’s website instantly, but you must use an username and password that is specific to the portal, even if you have been ordering from their standard web site. If you don’t, you will not receive the discounted pricing below, and you will be charged shipping. When placing your first order, you will see incorrect pricing and shipping charges, but your bill will be correct. NGS items cannot be ordered through the portal, but can be ordered via quotes.
Qiagen Account creation
Create an account on the Qiagen website. Place all of your items in a shopping cart. Proceed to check out, and use the “transfer cart” option to send the cart to customorders@jhmi.edu. We will place the order for you. Note: only items not available on the CoreStore website should be ordered using this method.
ThermoFisher Account Creation
You must link a ThermoFisher.com account to our portal to place orders. To create a new account or link an existing account, download and follow these instructions.
Use of the legacy ThermoFisher ordering tool has been discontinued.
Please set up a ThermoFisher portal account using the instructions linked above. If you need to place an order prior to your account set up being completed, please contact us.
Location
Johns Hopkins East Baltimore Medical Campus
600 N. Wolfe Street
Blalock Building, Room 1004
Baltimore, MD 21287 USA
Hours
Monday – Friday:
7:00 am to 4:30 pm
After-hours pickup is available from the lockers near the Blalock elevators. Please contact us to make arrangements.
Frequently asked questions
- For Horizon Discovery (Dharmacon), if you have a current account, you do not need to create a new account. Simply starting on our portal login page will associate your account with our portal. If you need to create an account, you can do so on the registration page. Orders created on the portal create a quote which is emailed to the GRCF. The GRCF will place the orders by the end of the day. You will receive an email when the order has been placed.
- For IDT, you must create a portal specific account, but account set-up is instantaneous. Simply go to our portal and create a new account. Make sure you do not click anywhere else on the page prior to creating the account, or the web-site will lose the association with our portal. Once you have set up the account, you may place your order. When placing your first order on a newly created account, you will see incorrect pricing and shipping charges, but your bill will be correct.
- For MilliporeSigma, you need to email us the following information:
- Your name
- Your email address
- Your phone number
- Your building and room number (for CoreStore deliveries)
- Your PI’s name & email address
- If you already have set up a GRCF account with MilliporeSigma, you may order using this link.
- Qiagen does not require an account. Shopping carts are emailed to the GRCF ( customorders@jhmi.edu) for ordering.
- For ThermoFisher you must link your ThermoFisher account to the Dashboard. Please download and follow these instructions.
Dharmacon and IDT are instantaneous. If you already have a Dharmacon direct account, you may simply login on our portal page and your orders will be associated with the portal. If you already have a direct IDT account, you MUST create a new account by clicking “Set up new account” on our portal page (without clicking anywhere else on the page.)
For MilliporeSigma, you must create an account and wait for an email indicating that the account is active. This may take up to two business days.
For ThermoFisher, you must link a ThermoFisher.com account to our portal. This process may take 24 hours.
Qiagen does not require an account. Orders are emailed to us and placed by the GRCF staff.
For IDT and MilliporeSigma, you must create an account specific to the GRCF portal in order to take advantage of our pricing. Existing ThermoFisher accounts may be used, once they are linked to the portal. Prior Horizon Discovery (Dharmacon) accounts work, as long as you start on our portal page.
On any of these sites, you will pay with your budget number. You do not need a credit card or a PO number from SAP. KKI customers should enter the last name of the PI on their grant or a KKI PO number. All customers (KKI and JHU) will be billed through iLab. JHU customers should check to make sure their budget numbers are active in iLab. Non-sponsored budget numbers must be added to iLab manually by following these instructions.
Standard oligo orders placed before noon from MilliporeSigma arrive the next business day. Standard oligo orders placed with IDT before 2 PM or ThermoFisher before 5 PM, will arrive in our office two business days after they are placed. Other items vary depending on the time it takes the manufacturer to ship. All items are available in Blalock 1004 the day they are received and will go out for delivery with the CoreStore the next morning.
No. Due to the sensitive nature of some of the items ordered through our portals, someone must be present in your lab for your order to be delivered.
Yes. On a typical day, we receive the orders by 11:30 AM. Orders are available in Blalock 1004 from the time they arrive until we close at 4:30 PM. You may stop by and pick them up anytime during those orders. If you call ahead, please be prepared to tell us the order number and then name of the person placing the order. Orders are brought to the CoreStore early the following morning and set out for delivery.
If you know that you would like to pick up your order at the time you place your order, you may replace your building and room number with the words “Pick up” and we will contact you when the order arrives. Pickup orders are placed in one of our lockers near the Blalock elevators and are available 24/7.
You should create a separate ordering account for each lab to make sure that your orders are delivered to the correct location.
This usually means that you have logged into the portal using a username and password that were set up for the SAP punchout or the normal IDT web-site. Using the wrong login will take you to the direct website without any notification. You will only receive free shipping if you use a username and password set up specifically on our portal. You will know you are on the correct website if you see a banner that says “You are using the DNA Analysis Facility Portal” on every page. Also, when you begin the checkout process, the billing and shipping information should be prepopulated with the GRCF address and PO. Click past this page and you will come to a page that asks for your budget number and building and room number.
All NGS products, including o-pools, must be ordered via custom quote. Please send your quote request to Roxann Ashworth, and she will work with IDT to create your quote and place your order. This will allow you to receive a small discount and free shipping.
