Next-generation Cas9 proteins for maximum editing efficiency
We offer two performance-leading Cas9 proteins to better meet your genome-editing goals— Invitrogen TrueCut Cas9 Protein v2, for most common research applications, and Invitrogen TrueCut HiFi Cas9 Protein, for applications that are sensitive to off-target effects. We also offer a Cas9 protein designed for clinical research that requires a Cas9 nuclease that is manufactured with higher stringency specifications.
Ordering
You must have a ThermoFisher.com account linked to the GRCF portal in order to use the portal. If you have not already done this, please download the instructions and create a new account or link your existing account to the portal. This process can take up to 24 hours.
Check out all of our CRISPR tools
We have developed tools and solutions for every step in the CRISPR genome editing workflow. The combined tool set comprises a collection of optimized, validated systems designed to work together to help answer your important scientific questions—faster and with less effort. Our portfolio is built on 30 years of industry-leading innovation and can grow with your research needs.
TrueCut Cas9 Protein v2
Intended for maximum editing efficiency: Consistently high on-target editing efficiency, up to 2X higher than the competition, in all tested cell lines, including standard, primary, stem, and immune (>90% editing in T-cells).
Choose from our catalog of ready-to-transfect predesigned sgRNAs for efficient knockout or design your own using one of our ordering tools. Recommended for use with the TrueCut Cas9 Protein v2.
Access genome editing application resources for more success as you plan and execute your experiments. These resources include protocols, videos, and publications.
TrueCut HiFi Cas9 Protein
Intended for improved specificity: Significantly reduce off-target effects in a broad range of cell types including primary, stem, and immune cells.
Choose your gRNA from our catalog of pre-designed gRNAs packaged as ready-to-use lentivirus. Available as individual constructs or screening libraries. Custom services also available.
A free online tool that enables scientists of all experience levels to easily design, select, and order reagents for accurate and successful gene editing experiments.
CTS TrueCut Cas9 Protein
Intended for clinical research: Designed under high stringency manufacturing and extensive safety testing. It provides consistently superior performance in all tested cell lines, including >90% editing efficiency in T cells and induced pluripotent stem cells.
The Precision gRNA Synthesis Kit is a complete system for rapid synthesis of guide RNA (gRNA) ready to complex with TrueCut™ Cas9 Protein v2 for transfection-ready Cas9 protein/gRNA ribonucleoprotein (Cas9 RNP).
Thermo Fisher Scientific is applying the power of the CRISPR-Cas9 system to high throughput screening applications with our new awarding winning pooled and arrayed CRISPR libraries. These CRISPR libraries are designed to provide you with a flexible system that adapts to your needs and your screening goals.
Frequently asked questions
Ordering lentivirus vectors from this site requires that investigators have a current recombinant DNA and infectious agent registration for this material from the Johns Hopkins Biosafety Office. The other components do not have any special requirements.
Since CRISPRs have been shown to have a wide variation in their activity, even when designed in close proximity to each other, it is recommended you order at least 2 gRNAs per target.
Standard oligo orders placed before noon from MilliporeSigma arrive the next business day. Standard oligo orders placed with IDT before 2 PM or ThermoFisher before 5 PM, will arrive in our office two business days after they are placed. Other items vary depending on the time it takes the manufacturer to ship. All items are available in Blalock 1004 the day they are received and will go out for delivery with the CoreStore the next morning.
No. Due to the sensitive nature of some of the items ordered through our portals, someone must be present in your lab for your order to be delivered.
Yes. On a typical day, we receive the orders by 11:30 AM. Orders are available in Blalock 1004 from the time they arrive until we close at 4:30 PM. You may stop by and pick them up anytime during those orders. If you call ahead, please be prepared to tell us the order number and then name of the person placing the order. Orders are brought to the CoreStore early the following morning and set out for delivery.
If you know that you would like to pick up your order at the time you place your order, you may replace your building and room number with the words “Pick up” and we will contact you when the order arrives. Pickup orders are placed in one of our lockers near the Blalock elevators and are available 24/7.
You should create a separate ordering account for each lab to make sure that your orders are delivered to the correct location.
