RNA Interference (RNAi) with Horizon/Dharmacon
Horizon/Dharmacon RNAi products encompass the most complete portfolio of innovative tools for transient, long-term, inducible and in vivo RNAi applications.
Please note: In order to ensure correct routing and delivery of your order, you must provide the following information in the “Order Notes” field in your shopping cart during checkout: Building & Room Number, PI Name & Email address
siRNA Products
Custom siRNA can be ordered through the siDESIGN Center
The siDESIGN Center is a free online design tool that offers the power of the Dharmacon SMARTselection algorithm for the custom design of highly functional siRNAs.
ON-TARGETplus siRNA incorporates design strategies for functionality and specificity, and is the only siRNA available with a patented dual-strand modification pattern to reduce off targets caused by both strands of the siRNA.
Blocks sense-strand entry into RISC and improves antisense-strand specificity to its target with a unique seed- region modificationDelivers optimal siRNA potency while eliminating off-targets that could cause false positivesPooling ON-TARGETplus-modified siRNAs results in the most specific gene targeting reagent
siGENOME siRNAs are highly potent and reliable gene-silencing reagents. Since its launch in 2004, siGENOME has provided the longest standing siRNA guarantee on the market.
siGENOME designs incorporate rational strand bias with application of ON-TARGET modifications when required for optimal functionalityTrusted in more whole genome screens than any other siRNA
Horizon/Dharmacon siRNA reagents are available in the following formats:
- SMARTpool: A mixture of four siRNAs provided as a single reagent. siGENOME and ON-TARGETplus SMARTpool reagents are guaranteed to silence by 75% or better.
- Set of Four: Four unique siRNAs targeting a single gene provided as individual reagents. Three of our four siGENOME and ON-TAREGETplus siRNAs are guaranteed to silence by 75% or better.
- Individuals: Select one, two, three, or four individual siRNAs per gene at a variety of quantities Gene Family and Pathways: Predefined libraries arranged by gene family and/or function. Custom Library: Available in 96-well or 384-well microtiter plates.
Please note: In order to ensure correct routing and delivery of your order, you must provide the following information in the “Order Notes” field in your shopping cart during checkout: Building & Room Number, PI Name & Email address
shRNA Products
SMARTvector Lentiviral shRNA –The SMARTchoice platform permits the researcher to first observe activity of multiple promoters in the target cells of interest and then order SMARTvector gene-specific lentiviral shRNA and controls with optimal promoter choices, saving time and money by making informed decisions.
SMARTvector Inducible Lentiviral shRNA – Combining all of the advantages of the SMARTvector shRNA design innovations with the recently developed Tet-On 3G tetracycline-inducible expression system and the flexibility of SMARTchoice promoter and reporter options, this novel single-vector regulatable RNAi system permits the rapid development of stable cells with tightly controlled shRNA expression.
GIPZTM Lentiviral shRNA – Perform specific and efficient, transient or long-term RNAi with GIPZ Lentiviral shRNA. Human and mouse genes are each targeted with multiple shRNA constructs that have been cloned into the pGIPZ lentiviral vector.
TRIPZTM Inducible Lentiviral shRNA – Study the function of essential genes, validate cellular phenotypes and generate regulatable knockdown cell lines with TRIPZ Inducible Lentiviral shRNA.
TRC Lentiviral shRNA – The RNAi Consortium (TRC) Lentiviral shRNA Library was developed by the Broad Institute of MIT and Harvard University. This collection targets both human and mouse genes with broad coverage per transcript.
GE Dharmacon shRNA reagents are available as:
Please note: In order to ensure correct routing and delivery of your order, you must provide the following information in the “Order Notes” field in your shopping cart during checkout: Building & Room Number, PI Name & Email address
