Nucleic Acid Quality Assessments

Fragment Analyzer Services

Nucleic Acids Quality Number

Fragment Analyzer PROSize software determines a RQN (RNA Quality Number) of total RNA on a scale from 1 to 10 that correlates to current industry practices (RIN). 

For more information:  Comparison of RIN and RQN 


DNA PEAK SIZE vs. SMEAR ANALYSIS

DNA size can be reported as a peak size or smear size. The peak size is the tallest/mot concentrated portion of the sample, while the smear size accounts for the entire distribution of the sample over the designated smear range. 

Fragments and smears have different separation profiles on electropherograms. Fragments are displayed as sharp peaks, while smears are broader and lower in height than fragments with the same concentration. 

More information about best sizing practice, here.


DV200 

For RNA FFPE samples, DV200 is a reliable predictor of successful RNA sequencing. More information, here. 


We offer the following Agilent kits:

Sample Requirements and Shipment

DNA Samples RNA Samples Sample Shipment
DNF-488 Genomic DNA Kit Information
  • Sizing Range: 50 bp – 20,000 bp
  • gDNA Concentration Range: 50 pg/µL – 5 ng/uL input gDNA

DNF-474 HS NGS Fragment Kit Information
  • Sizing Range: 100 bp – 6,000 bp
  • Fragment Concentration Range: 5 pg/μL to 500 pg/μL input DNA
  • Smear Concentration Range: 50 pg/μL to 5,000 pg/μL input DNA


Sample Volume

Send a minimum of 5uL  per sample for any Agilent kit of your preference

DNF-467 Genomic DNA 50 kb Kit Information
  • Sizing Range: 75 bp – 50,000 bp
  • gDNA Concentration Range: 25 ng/µL – 250 ng/uL input gDNA


DNF-473 NGS Fragment Kit Information
  • Sizing Range: 100 bp – 6,000 bp
  • Fragment Concentration Range: 0.1 ng/μL to 10 ng/μL input DNA
  • Smear Concentration Range: 5 ng/μL to 100 ng/μL input DNA


DNA Quantification
  • If you do not know the concentration of your sample, please request Qubit Analysis and we will determine it for you.
  • Submit a minimum of 10uL DNA if not supplying DNA with a concentration.


More Questions

Please contact us if you have any further question for sample submission or shipment. 

DNF-472 HS RNA kit Information
  •  Sizing Range: 200 nt – 6,000 nt. 
  • Total RNA Input Concentration Range: 50 pg/µL – 5,000 pg/uL
  • mRNA Input Concentration Range: 250 pg/μL – 5,000 pg/μL 


Sample volume

Send a minimum of 5uL  per sample for any Agilent kit of your preference.

RNA Quantification
  • If you do not know the concentration of your sample, please request Qubit Analysis and we will determine it for you.
  • Submit a minimum of 10 uL RNA if not supplying RNA with a concentration.


More Questions

Please  contact us if you have any further question for sample submission or shipment.

On-Site Customers 

Drop off samples in our lab by 4 PM on Monday for inclusion in that week’s processing schedule. 

  • For DNA samples: Drop off samples at RT or with ice packs, if samples are frozen or at -80 dry ice is recommended.
  • For RNA samples: Drop off samples at PCTB G20 on dry ice.
  • Please follow all the instruction in iLab for sample submission and shipment.


Off-Side Customers

Ship samples to our lab to be received by Tuesday morning for inclusion in that week’s processing schedule.

  • For DNA samples: Ship at ambient temperature or with ice packs. If samples are frozen or at -80C, ship them on dry ice.
  • For RNA samples: Ship on dry ice.  
  • Please follow all the instruction in iLab for sample submission and shipment.


Turnaround Time

One week. If a sample needs to be repeated it will be included the next time the assay is run. 


Our Location

Nucleic Acid Technologies Lab
Johns Hopkins University
725 N. Wolfe Street
PCTB G20
Baltimore, MD 21205

More Questions

Please  contact us if you have any further question for sample submission or shipment.

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