Delivering CRISPR at any scale: from research to GMP.
Partner with Agilent & the GRCF to benefit from industry leading nucleic acid solutions and efficiently advance from research to lead oligo candidates for clinic and market. One of the many advantages of working with Agilent is their ability to provide a seamless experience from research grade to GMP material. Extensive knowledge and experience enable Agilent’s nucleic acid experts to provide high purity R&D grade material for your preliminary research assays and development phase pilots, and then accelerate the scale-up to large quantity GMP material when you are ready.
Ordering
To start the order process, fill out the custom quote spreadsheet linked below and email it to the GRCF.
Note: Ordering lentivirus vectors from this site requires that investigators have a current recombinant DNA and infectious agent registration for this material from the Johns Hopkins Biosafety Office. The other components do not have any special requirements.
SureGuide CRISPRs
Agilent’s SureGuide Chemically Synthesized sgRNAs for CRISPR are the industry standard for quality nucleic acids. Using a patented, proprietary chemistrymakes the synthesis of long RNA oligonucleotides as robust and efficient as DNA synthesis. This chemistry is used to synthesize and deliver ultra-high-quality CRISPR guides that have few contaminants. Agilent developed several unique chemical modifications that improve the efficiency, stability and specificity of guide RNAs in a variety of cell types.
Agilent supports you through all phases of growth and will assist in the transition from early stage research to clinical development to commercialization.
Custom sequences up to 140 nt, longer lengths available upon request
Quantities from 100 µg to 100 mg (3 – 3000 nmol) to scale your experiment
Research and Development grade to GMP material
Chemical modifications at no extra charge (M or MS at 5′ and 3′ ends)
Additional modifications and longer lengths available upon request, ensuring the flexibility you need.
Frequently asked questions
Ordering lentivirus vectors from this site requires that investigators have a current recombinant DNA and infectious agent registration for this material from the Johns Hopkins Biosafety Office. The other components do not have any special requirements.
Since CRISPRs have been shown to have a wide variation in their activity, even when designed in close proximity to each other, it is recommended you order at least 2 gRNAs per target.
Standard oligo orders placed before noon from MilliporeSigma arrive the next business day. Standard oligo orders placed with IDT before 2 PM will arrive in our office two business days after they are placed. Other items vary depending on the time it takes the manufacturer to ship. All items are available in Blalock 1004 the day they are received and will go out for delivery with the CoreStore the next morning.
No. Due to the sensitive nature of some of the items ordered through our portals, someone must be present in your lab for your order to be delivered.
Yes. On a typical day, we receive the orders by 11:30 AM. Orders are available in Blalock 1004 from the time they arrive until we close at 4:30 PM. You may stop by and pick them up anytime during those orders. If you call ahead, please be prepared to tell us the order number and then name of the person placing the order. Orders are brought to the CoreStore early the following morning and set out for delivery.
If you know that you would like to pick up your order at the time you place your order, you may replace your building and room number with the words “Pick up” and we will contact you when the order arrives. Pickup orders are placed in one of our lockers near the Blalock elevators and are available 24/7.
