The GRCF is proud to offer Whole Genome Sequencing on the Novaseq Xplus

Human Whole Genome Sequencing

Human genome sequencing at 30x average coverage, from short-insert paired-end reads using a single library.

Input DNA Requirement: 1ug high molecular weight genomic DNA for PCR free (preferred), 500ng for PCR based methods

Note: ordering server is only accessible to internal JHU customers.  External customer should contact David Mohr to place their orders.

We have experience with non-human whole genome sequencing and analysis, as well.  Contact David Mohr for more information.

Price per Sample

$650*

*Saliva samples require a 20% additional charge

Deliverables:

  • Genomic coverage at a minimum of 30x
  • Single sample, multi-sample, and trio de novo calling using Illumina’s DRAGEN pipeline
  • SNPS/Indels, Structural Variants, and CNV files reported in VCF format
  • Annotation using Annovar and AnnotSV
  • Concordance with whole-genome genotyping arrays
  • Raw data (reads and quality scores) and analyzed data in industry-standard format (archival BAM)
  • Comprehensive QC Report