Fragment Analyzer Services

Nucleic Acids Quality Number

Fragment Analyzer PROSize software determines a RQN (RNA Quality Number) of total RNA on a scale from 1 to 10 that correlates to current industry practices (RIN).

For more information: Comparison of RIN and RQN

DNA Peak Size vs. Smear Analysis

DNA size can be reported as a peak size or smear size. The peak size is the tallest/mot concentrated portion of the sample, while the smear size accounts for the entire distribution of the sample over the designated smear range.

Fragments and smears have different separation profiles on electropherograms. Fragments are displayed as sharp peaks, while smears are broader and lower in height than fragments with the same concentration.

More information about best sizing practice, here.

DV200

For RNA FFPE samples, DV200 is a reliable predictor of successful RNA sequencing. More information is available here.

We offer the following Agilent kits:

Sample Requirements and Shipment

DNA Samples

DNF-488 Genomic DNA Kit Information

  • Sizing Range: 50 bp – 20,000 bp
  • gDNA Concentration Range: 50 pg/µL – 5 ng/uL input gDNA

DNF-467 Genomic DNA 50 kb Kit Information

  • Sizing Range: 75 bp – 50,000 bp
  • gDNA Concentration Range: 25 ng/µL – 250 ng/uL input gDNA

RNA Samples

DNF-474 HS NGS Fragment Kit Information

  • Sizing Range: 100 bp – 6,000 bp
  • Fragment Concentration Range: 5 pg/μL to 500 pg/μL input DNA
  • Smear Concentration Range: 50 pg/μL to 5,000 pg/μL input DNA

DNF-473 NGS Fragment Kit Information

  • Sizing Range: 100 bp – 6,000 bp
  • Fragment Concentration Range: 0.1 ng/μL to 10 ng/μL input DNA
  • Smear Concentration Range: 5 ng/μL to 100 ng/μL input DNA

Sample Shipment

Sample Volume

Send a minimum of 5uL per sample for any Agilent kit of your preference

DNA Quantification

  • If you do not know the concentration of your sample, please request Qubit Analysis and we will determine it for you.
  • Submit a minimum of 10uL DNA if not supplying DNA with a concentration.

More Questions

Please contact us if you have any further question for sample submission or shipment.

Ordering

Ready to order? Fill out a request on our iLab ordering site.
New to iLab? See the instructions found in the ordering menu above.