Gene Expression and MicroRNA Arrays

Taqman Gene Expression Arrays may be ordered and run through the GRCF

Applied Biosystems by ThermoFisher’s OpenArray chips are ideal for investigators who wish to look at multiple Taqman assays on medium to large numbers of samples. They come in fixed and custom content options. MicroRNA profiling can also be done using this method.

Understanding the OpenArray

The technology spots Taqman assays onto chips, which are then run in our QuantStudio 12K flex machine.

The number of samples on each chip varies, based on fixed content or custom option chosen.

Custom chips can be formatted with 18, 56, 112, 168 or 224 assays.

There is a minimum order of 10 custom chips, but smaller projects are often cost effective, even when excess chips must be ordered.

Custom Array Pricing

Pricing varies by number of gene expression assays and sample numbers. Chips which use custom design assays are more expensive then chips that use only inventoried assays.

Examples using minimum numbers, for both options, are given below, but please contact us for detailed information.

Please note that if you would run your samples in triplicate in a standard gene expression assay, you should do so with these assays as well. Sample numbers given below do not take that into consideration.

Chip Format	# Samples/Chip	Total Samples	Price	Total cost**
18 Assays*	48	480*	Chips: $15,380 Other costs: $1950	$17,330
56 Assays	48	480	Chips: $17,205 Other Costs: $1950	$19,155
112 Assays	24	240	Chips: $18,270 Other costs: $3,900	$22,170
168 Assays	16	160	Chips: $20,260 Other costs $5,850	$26,110
224 Assay	12	120	Chips: $21,045 Other costs: $7,800	$28,845

*18 assay formatted shapes are plated in triplicate, so each sample is used once, but run 3 times. The formats are single sample numbers, not run in triplicate.

How do I submit my samples?

Customers should prepare cDNA from total RNA, following recommended procedures, and fill out our online request form. Prepared cDNA should be brought to Blalock 1004 or dropped off in one of our remote locations in the appropriate number of 96 well plates. Mark each plate with the order number from the online request. Data will be returned to you via OneDrive.

Location

Johns Hopkins East Baltimore Medical Campus
600 N. Wolfe Street
Blalock Building, Room 1004
Baltimore, MD 21287 USA

Hours

Monday – Friday:
7:00 am to 4:30 pm

After-hours drop-off is available in the refrigerator outside Blalock 1004. Please ask us for the freezer combination when preparing to drop off this sample type.

Contact

[email protected]

410-955-2836

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