The GRCF is proud to offer whole genome sequencing on the NovaSeq 6000
Human Whole Genome: $650 per genome*
Human genome sequencing at 30x average coverage, from short-insert paired-end reads using a single library.
Input DNA Requirement: 1ug high molecular weight genomic DNA for PCR free (preferred), 500ng for PCR based methods
Deliverables:
Genomic coverage at a minimum of 30x
Single sample, multi-sample, and trio de novo calling using Illumina’s DRAGEN pipeline
SNPS/Indels, Structural Variants, and CNV files reported in VCF format
Annotation using Annovar and AnnotSV
Concordance with whole-genome genotyping arrays
Raw data (reads and quality scores) and analyzed data in industry-standard format (archival BAM)
Comprehensive QC Report
*(studies with low sample numbers may increase pricing)
Whole Genome Analysis: $50 per sample
We now offer analysis of previously generated whole genome data, including mapping, multisample calling, and annotation. Cost is $50/sample. More details below. Thanks to lower pricing of v1.5 reagents, we now offer lower whole genome pricing.
Input Requirements: demultiplexed fastq or BAM/CRAM
Deliverables:
Single sample, multi-sample, and trio de novo calling using Illumina’s DRAGEN pipeline
SNPS/Indels, Structural Variants, and CNV files reported in VCF format
Single sample, multi-sample, and trio de novo calling using Illumina’s DRAGEN pipeline
Annotation using Annovar and AnnotSV
Comprehensive QC Report
