cDNA Synthesis

cDNA Synthesis for MicroRNA Profiling and Custom Gene Expression Taqman Arrays

The GRCF offers cDNA synthesis to support customers requesting:

 

Pricing

How to Order

Ordering

Please contact FAF for cDNA synthesis ordering.

Please contact  Roxann Ashworth [410-614-0702] for Taqman OpenArray, MicroRNA Profiling Analysis, or custom and fixed content Gene Expression Array analysis on the QuantStudio 12K Flex Real-Time PCR System .

RNA Requirements

• RNA should be free of PCR inhibitors
• Has a A260/230 ratio between 2.0 and 2.4
• Has a A260/280 ratio between 1.8 and 2.1
• Has a RNA Integrity Number (RIN) or RNA Quality Number (RQN) between 6.5 and 10
• The recommended amount of total RNA for preparing cDNA for microRNA Profiling is 100ng.
• The recommended amount of total RNA for preparing cDNA for Gene Expression Taqman Arrays is 10µl at 200ng/µl (2µg).
• Qualitative analysis of RNA samples is available by  Advanced Analytical Fragment Analyzer CE analysis. The system determines a RQN (RNA Quality Number) of total RNA on a scale from 1 to 10 that correlates to current industry practices (RIN).

Questions

cDNA Synthesis Questions: Please contact the NAT Team.

Please contact Roxann Ashworth ([email protected]) for all other questions including: 

Taqman OpenArray, MicroRNA Profiling Analysis or Gene Expression Array analysis on the QuantStudio 12K Flex Real-Time PCR System