Genetic Resources Core Facility (GRCF)

Assays Galore: qPCR, dPCR, SNP & More

IDT

Featuring PrimeTime gene Expression assays and the rhAmp Genotyping system

Qiagen

Featuring Quantinova probe and PCR assays, dPCR assays and SNP detection assays

ThermoFisher Scientific

Featuring Taqman Assays for Gene Expression, SnP and Copy number Variation

dPCR/qPCR Assays

Differences and changes in gene expression are important measures to understand biological systems. One of the most widely used methods of analyzing gene expression is quantitative PCR (qPCR) following cDNA synthesis. qPCR, also called real-time PCR, uses a fluorescent reporter molecule with a primer pair to measure target nucleic acid amplification during PCR cycling. Most qPCR assays can also be used for dPCR

PrimeTime Assays from IDT

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Our partner companies offer a variety of tools for performing qPCR, including off the shelf assays (both probe based and intercalating dye based) and custom assays. PrimeTime qPCR assays come in two formats: probe based assays and primer only assays.

Probe based assays may be ordered with a variety of dye and quencher combinations, in three different scales: 100, 500 or 2500 reactions. Predesigned human, mouse and rat assays are available, and IDT also provides tools to help with the design of custom assays.
Primer only assays are ideal for using with intercalating dye experiments. Each order of these primers is enough for 500 reactions.
Primer and probe sequences are provided with all orders.

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QuantiNova Assays from Qiagen

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QuantiNova LNA assays come in two formats: probe based assays and primer only assays.

Choose from the broadest selection of predesigned assays for accurate and sensitive detection of any human, mouse or rat mRNA or lncRNA – from general transcript detection to differentiation of specific transcript isoforms. LNA enhancement allows the design of shorter primers and probes, which are more flexible to position on the target, providing opportunities to design for specific transcript detection or target differentiation. Plus, thorough design validation ensures optimal performance and robust detection, and assays for all popular mRNAs and lncRNAs undergo wet-lab validation.

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Taqman Assays from ThermoFisher

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TaqMan Gene Expression assays are built on more than 20 years of real-time PCR gene expression experience. Each assay includes target primers and a sequence-specific probe optimized for the best functional performance. No additional design, optimization, or melt curve analysis is needed. Available in a wide variety of formats and species, new assay designs are constantly added to help meet your research needs. TaqMan assays have been cited in over 40,000 publications and are backed by more than 350 patents.

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A few things to know

Specialized quotes from must be handled outside of the portal through contact with the local sales representative and the GRCF. Please email us with the details of what you would like to order.

IDT Account Creation

You can create an account on IDT’s website instantly, but you must use an username and password that is specific to the portal, even if you have been ordering from their standard web site. If you don’t, you will not receive the discounted pricing below, and you will be charged shipping. When placing your first order, you will see incorrect pricing and shipping charges, but your bill will be correct. NGS items cannot be ordered through the portal, but can be ordered via quotes.

Qiagen Account Creation

Create an account on the Qiagen website. Place all of your items in a shopping cart. Proceed to check out, and use the “transfer cart” option to send the cart to [email protected]. We will place the order for you. Note: only items not available on the CoreStore website should be ordered using this method.

ThermoFisher Account Creation

You must link a ThermoFisher.com account to our portal to place orders. To create a new account or link an existing account, download and follow these instructions.

Use of the legacy ThermoFisher ordering tool has been discontinued.

Please set up a ThermoFisher portal account using the instructions linked above. If you need to place an order prior to your account set up being completed, please contact us.

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