Nucleic Acid Quality Assessments
Fragment Analyzer Services
Fragment Analyzer PROSize software determines a RQN (RNA Quality Number) of total RNA on a scale from 1 to 10 that correlates to current industry practices (RIN).
more informationDNA PEAK SIZE vs. SMEAR ANALYSIS
DNA size can be reported as a peak size or smear size. The peak size is the tallest/mot concentrated portion of the sample, while the smear size accounts for the entire distribution of the sample over the designated smear range.
Fragments and smears have different separation profiles on electropherograms. Fragments are displayed as sharp peaks, while smears are broader and lower in height than fragments with the same concentration.
DV200
For RNA FFPE samples, DV200 is a reliable predictor of successful RNA sequencing. More information, here.
We offer the following Agilent kits:
DNA:
NGS:
RNA:
Sample Requirements and Shipment
DNA Samples
DNF-488 Genomic DNA Kit Information
Sizing Range: 50 bp – 20,000 bp
gDNA Concentration Range: 50 pg/µL – 5 ng/uL input gDNA
DNF-474 HS NGS Fragment Kit Information
Sizing Range: 100 bp – 6,000 bp
Fragment Concentration Range: 5 pg/μL to 500 pg/μL input DNA
Smear Concentration Range: 50 pg/μL to 5,000 pg/μL input DNA
DNF-467 Genomic DNA 50 kb Kit Information
Sizing Range: 75 bp – 50,000 bp
gDNA Concentration Range: 25 ng/µL – 250 ng/uL input gDNA
DNF-473 NGS Fragment Kit Information
Sizing Range: 100 bp – 6,000 bp
Fragment Concentration Range: 0.1 ng/μL to 10 ng/μL input DNA
Smear Concentration Range: 5 ng/μL to 100 ng/μL input DNA
Sample Volume: Send a minimum of 5uL per sample for any Agilent kit of your preference
DNA Quantification
If you do not know the concentration of your sample, please request Qubit Analysis and we will determine it for you.
Submit a minimum of 10uL DNA if not supplying DNA with a concentration.
RNA Samples
DNF-472 HS RNA kit Information
Sizing Range: 200 nt – 6,000 nt.
Total RNA Input Concentration Range: 50 pg/µL – 5,000 pg/uL
mRNA Input Concentration Range: 250 pg/μL – 5,000 pg/μL
Sample Volume: Send a minimum of 5uL per sample for any Agilent kit of your preference.
RNA Quantification
If you do not know the concentration of your sample, please request Qubit Analysis and we will determine it for you.
Submit a minimum of 10 uL RNA if not supplying RNA with a concentration.
Sample Shipment
On-Site Customers
Drop off samples in our lab by 4 PM on Monday for inclusion in that week’s processing schedule.
For DNA samples: Drop off samples at RT or with ice packs, if samples are frozen or at -80 dry ice is recommended.
For RNA samples: Drop off samples at PCTB G20 on dry ice.
Please follow all the instruction in iLab for sample submission and shipment.
Off-Side Customers
Ship samples to our lab to be received by Tuesday morning for inclusion in that week’s processing schedule.
For DNA samples: Ship at ambient temperature or with ice packs. If samples are frozen or at -80C, ship them on dry ice.
For RNA samples: Ship on dry ice.
Please follow all the instruction in iLab for sample submission and shipment.
Turnaround Time
One week. If a sample needs to be repeated it will be included the next time the assay is run.
Our Location
Nucleic Acid Technologies Lab
Johns Hopkins University
725 N. Wolfe Street
PCTB G20
Baltimore, MD 21205
More Questions?
Please contact us if you have any further question for sample submission or shipment.
How do I get started?
Place your order in iLab, our secure online ordering system.
