The GRCF offers library preparation, sequencing and analysis of RNA seq data.
We offer simple, per lane pricing, which allows the investigator control over how many reads required per sample. Please see ENCODE’s best practices for current guidelines on RNASeq experimental design.
Library Prep Sample Requirements
Due to the nature of these experiments, it is beneficial to discuss sequencing strategy prior to sample submission.
High quality RNA
Please provide us with high quality total RNA (RIN >8.5)
>500 ng
We request >500ng for standard prep, but can work with less if required.
Pre-QC Testing
We strongly encourage you to QC your RNA prior to submission, as high quality starting material is the key to success.
QC Testing for a Fee
If you cannot QC your sample prior to submission, we can do so for you at a cost of $20/sample.
Sequencing Costs
Sequencing costs vary based on read length and depth per sample required. You can multiplex samples in a single lane to save costs on sequencing. Please see our High Throughput Sequencing page for information on per lane pricing and data yield.
Analysis
We collaborate with the JHU Computational Biology Consulting Core for RNASeq analysis.
