Our Portals
The GRCF DNA Services group offers the synthesis of oligonucleotides through Horizon Discovery, IDT, MilliporeSigma and ThermoFisher. Ordering through the Johns Hopkins University portal lets you take advantage of lower pricing than you will get through SAP. Most companies provide free shipping. Oligos can be delivered to your lab by the Core Store for free or picked up in our office in Blalock 1004.
Horizon Discovery (Dharmacon)
https://horizondiscovery.com/en/eprocurement/johns-hopkins-university-grcf
Integrated DNA Technologies (IDT)
https://www.idtdna.com/CoreInstitutions/Login.aspx?p=JohnsHopkins
Millipore Sigma
https://www.sigmaaldrich.com/US/en/login?redirect=%2FUS%2Fen
A few things to know
Specialized quotes from must be handled outside of the portal through contact with the local sales representative and the GRCF. Please email us with the details of what you would like to order.
IDT Account Creation
You can create an account on IDT’s website instantly, but you must use an username and password that is specific to the portal, even if you have been ordering from their standard web site. If you don’t, you will not receive the discounted pricing below, and you will be charged shipping. When placing your first order, you will see incorrect pricing and shipping charges, but your bill will be correct. NGS items cannot be ordered through the portal, but can be ordered via quotes.
MilliporeSigma Account creation
To set up an account with MilliporeSigma, please email us with the information listed below. Account set up takes about 24 hours.
Your name & email address
Your phone number
Your PI’s name & email address
Your JHU building and room number (for deliveries)
Horizon Discovery Account Creation
Horizon Discovery accounts can be created instantly. If you already have a direct account, you may use that account on the portal and do not need to set up a new account.ThermoFisher Account Creation
You must link a ThermoFisher.com account to our portal to place orders. To create a new account or link an existing account, download and follow these instructions.
Pricing:
The products listed below are a small sample of the products available from each vendor. Please explore the portals to see all of the products available, along with pricing.
Horizon Discovery’s pricing is based on the exact design of the oligo ordered and does not follow the per base model. Generally speaking they are competitive in the highly modified oligo arena, especially for RNA oligos and RNA:DNA hybrid oligos.
IDT
$0.18 per base
25 nmole scale; 3.0 OD min. yield
15-60 bases, no purification
Order by 1pm for free next day shipping*
100 nmole: $0.26/base; 10-90 bases, 6.0 OD min. yield
250 nmole: $0.72/base; 5-100 bases; 15.0 OD min. yield
1 umol: $1.19/base; 5-100 bases; 45.0 OD min. yield
Larger scales available; Same day shipping, purification and/or modifications add cost.
Free shipping on all products; orders placed before 1 PM ship the next day.*
NGS items must be ordered through a custom quote. Contact us for details. For pricing on other IDT products, please see the website.
DNA oligos are short, single- and double-stranded synthetic DNA sequences that can be used in nearly any molecular biology application. Using the Oligo Entry ordering tool, you can design your oligos to contain any DNA sequence you require and select from hundreds of modifications to suit your specific research needs.
Every DNA oligo you receive will be deprotected and desalted to remove small molecule impurities. In addition, your oligos will be quality controlled via proprietary ESI-mass spectrometry methods *, and quantified twice by UV spectrophotometry to provide accurate yield measurements.
MilliporeSigma
$0.15 per base
25 nm scale; 3.0 OD min. yield
2-60 bases, no purification
Free same day shipping on orders placed before 1pm*
50 nmole: $0.22/base; 2-120 bases; 5.0 OD min. yield
200 nmole: $0.53/base; 2-120 bases; 12.0 OD min. yield
1 umole: $0.95/base; 2-120 bases; 40 OD min. yield
Larger scales available; purification and/or modifications add cost.
Free shipping on all products; Orders placed before 1 PM ship same day for the same price with no shipping charges.*
si/shRNA products may also be ordered through this site.
DNA oligo products including primers, qPCR probes, high-throughput DNA in plates and next-generation sequencing (NGS) adapters available in multiple formats and manufactured under ISO 9001 and ISO 13485 quality systems for research or commercial applications.
Every DNA product is quality checked using MALDI-TOF and Electrospray Ionization (ESI) Mass Spec and OD by UV spectroscopy. Shipments include a quality assurance document for your records. Choose from our comprehensive portfolio of predesigned and custom products available in multiple formats.
ThermoFisher Scientific
$0.14 per base
25 nm scale; 2.0 OD min. yield
2-100 bases, no purification
Order by 5pm for free next day shipping*
50 nmole: $.20/base; 20-100 bases, 4 OD min. yield
200 nmole: $0.70/base; 20-100 bases, 16 OD min. yield
1 umole: $2.00/base, 50 OD min. yield
Larger scales available; Same day shipping, purification and/or modifications add cost.*
Free shipping on all products.
Taqman assays, CRISPR gRNAs and other custom products may also be ordered through this site.
Our oligos are available in a range of synthesis scales, purification options, and modifications. Many different applications demand different scale or purity to work well when it comes to custom DNA oligo synthesis.
Our custom DNA oligos are synthesized on a highly automated, computer-controlled system using standard cyano-ethyl phosphoramidite chemistry. Coupling efficiencies are monitored throughout synthesis of each oligonucleotide by trityl analysis, ensuring the quality of the process, not just the end product. Post-synthesis QC, mass spectrometry for short oligos and capillary electrophoresis (CE) for long oligos, ensure quality.
*Maximum oligo length, scale, purification type and modification type restrictions apply to same day/next day shipping standards. Please see the details on your favorite company’s portal before planning an experiment. Oligos will be delivered to your lab within 24 hours of receipt in Blalock; Pick up arrangements may be made by calling 410-955-2836 or emailing us.
Location
Johns Hopkins East Baltimore Medical Campus
600 N. Wolfe Street
Blalock Building, Room 1004
Baltimore, MD 21287 USA
Hours
Monday – Friday:
7:00 am to 4:30 pm
After-hours pickup is available from the lockers near the Blalock elevators. Please contact us to make arrangements.
Contact
Phone: 410-955-2836
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