The GRCF is proud to offer Whole Genome Sequencing on the Novaseq Xplus
Human Whole Genome Sequencing
Human genome sequencing at 30x average coverage, from short-insert paired-end reads using a single library.
Input DNA Requirement: 1ug high molecular weight genomic DNA for PCR free (preferred), 500ng for PCR based methods
Note: ordering server is only accessible to internal JHU customers. External customer should contact David Mohr to place their orders.
We have experience with non-human whole genome sequencing and analysis, as well. Contact David Mohr for more information.
Price per Sample
$650*
*Saliva samples require a 20% additional charge
Deliverables:
- Genomic coverage at a minimum of 30x
- Single sample, multi-sample, and trio de novo calling using Illumina’s DRAGEN pipeline
- SNPS/Indels, Structural Variants, and CNV files reported in VCF format
- Annotation using Annovar and AnnotSV
- Concordance with whole-genome genotyping arrays
- Raw data (reads and quality scores) and analyzed data in industry-standard format (archival BAM)
- Comprehensive QC Report