Pricing Tables
NovaSeq runs include additional cycles for dual indexed libraries.
| 1.5B | 10B | 25B |
|---|---|---|
| 100 cycle: $3,200 | 100 cycle: $8,400 | 100 cycle: $13,100 |
| 200 cycle: $3,700 | 200 cycle: $10,300 | |
| 300 cycle: $4,000 | 300 cycle: $10,900 | 300 cycle: $17,800 |
NovaSeq X Plus Lane Pricing
| 10B: ~2.5 billion reads per lane |
|---|
| 300 cycle: $1400 |
NovaSeq X Plus Lane Order
10B: ~2.5 billion paired reads, $1400
NovaSeq X Plus Flow Cell Order
1.5B: ~3 billion paired reads, 10B: ~20 billion paired reads, 25B: ~50 billion paired reads
Novaseq X Plus
We now offer runs on the new Novaseq X Plus platform. If you require NovaSeq 6000 please contact David Mohr for details
NovaSeq X
GRCF High Throughput Sequencing Center
Our goal is to provide the Johns Hopkins community access to Next Generation sequencing platforms.
We currently feature MiSeq and NovaSeq X Plus instruments. We do our best to sequence samples in a timely manner, but our primary focus is on generating high quality data.
Sample Requirements
Understanding Data Yield
Our facility features the NovaSeq platform. Yield is dependent upon several factors:
Read Length & Read Type
The longer the read, the more data. Paired end reads yield twice the data as single read.
Read Length & Read Type
The longer the read, the more data. Paired end reads yield twice the data as single read.
Optimal Cluster Density
It is imperative to accurately quantitate your library to ensure high data yield. We do our best to QC libraries before sequencing, but we cannot pool samples for you.
High Quality Library
Libraries that contain a high level of adapter dimers will yield significantly less data, particularly on the NovaSeq6000. Similarly, over amplified libraries can negatively impact yield
Please see Illumina’s NovaSeq X Specification page for current data yields. While we regularly achieve greater than ‘spec’ yields from the NovaSeq X, it is best to be conservative when planning your experiments.
Data Delivery
Per lane/flowcell sequencing
Data will be returned in Sanger FASTQ format via our high speed aspera server.
End to end services
Alignment files, variant calls, and any intermediate files you wish via aspera server.