Whole Genome Sequencing

The GRCF High Throughput Sequencing Center now offers whole genome sequencing on the NovaSeq 6000.

NEW: WGS Analysis, Updated Pricing

We now offer analysis of previously generated whole genome data, including mapping, multisample calling, and annotation.  Cost is $50/sample.  More details below. Thanks to lower pricing of v1.5 reagents, we now offer lower whole genome pricing

Human Whole Genome

Human genome sequencing at 30x average coverage, from short-insert paired-end reads using a single library.

Sample Requirements Input DNA amount: 1ug high molecular weight genomic DNA for PCR free (preferred), 500ng for PCR based methods
Deliverables
  • Genomic coverage at a minimum of 30x
  • Single sample, multi-sample, and trio de novo calling using Illumina’s DRAGEN pipeline
  • SNPS/Indels, Structural Variants, and CNV files reported in VCF format
  • Annotation using Annovar and AnnotSV
  • Concordance with whole-genome genotyping arrays
  • Raw data (reads and quality scores) and analyzed data in industry-standard format (archival BAM)
  • Comprehensive QC Report
Pricing $1100/genome (dependent upon sample number)

Whole Genome Analysis

Input Requirements demultiplexed fastq or BAM/CRAM
Deliverables
  • Single sample, multi-sample, and trio de novo calling using Illumina’s DRAGEN pipeline
  • SNPS/Indels, Structural Variants, and CNV files reported in VCF format
  • Single sample, multi-sample, and trio de novo calling using Illumina’s DRAGEN pipeline
    Annotation using Annovar and AnnotSV
  • Comprehensive QC Report
Pricing $50/sample
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