PCR Amplification Support Services

Pyrosequencing Projects

PCR amplification support for Pyrosequencing methylation studies are available to customers utilizing the GRCF Pyrosequencing service. We will amplify your samples using a customer supplied Qiagen predesigned or custom kit. Service includes PCR of samples, amplification confirmation by agarose gel electrophoresis and submission for Pyrosequencing. Please contact DNA Services or call 410-955-2836 to initiate a custom project.

Sample Requirements

Pyrosequencing (methylation analysis): 40 ng at 5 ng/ul to 10 ng/µl of bisulfite converted DNA per amplicon. If submitting genomic DNA for bisulfite conversion, we recommend converting a minimum of 100 ng per assay (amplicon).
Pyrosequencing (SNP analysis, mutation detection, sequencing): 40 ng at 5 ng/µl to 10 ng/µl per amplicon.

Sanger Sequencing Projects

For customers interested in custom Sanger sequencing projects we can help you with design of primers, development of PCR amplification conditions, amplification of samples, and sequence analysis. Please contact DNA Services or call 410-955-2836 to initiate a custom project.

Pricing

Pricing is project and sample number dependent.