At the GRCF High Throughput Sequencing Center, our goal is to provide the research community at Johns Hopkins University with access to ‘next generation’ sequencing platforms. We currently feature three HiSeq 2500 instruments and two MiSeq‘s and an Ion Proton.  We do our best to sequence samples in a timely manner, but our first priority is good data.

We offer simple, per lane pricing, along with end to end service for whole exome/custom targeted projects, RNASeq, and whole genome.

We offer free consultation. Please contact David Mohr to discuss your project in detail.

Per Run Pricing

MiSeq reagents are sold by cycle number.  They can be run in single/paired end, and/or indexed mode.

50 cycles (v2):$900 per run
150 cycles (v3):$1,000 per run
300 cycles (v2):$1,000 per run
500 cycles (v2):$1,100 per run
600 cycles (v3):$1,400 per run

Sample Requirements

Please submit 10μl of your sample at 2nM.  Samples must be pooled.  We will do a QC check via Bioanalyzer to increase the likelihood of quality data, but you should quantitate your sample as accurately as possible.  Nanodrop is not reliable.  qPCR is by far the most accurate, but intercalating dye methods can be used.

Data Yield

Data Yield is dependent upon several factors:

  • Read Length: the longer the read, the more data.
  • Read Type: paired end reads yield twice the data as single read.
  • Optimal Cluster density: it is imperative to accurately quantitate your library to ensure high data yield. We do our best to QC libraries before sequencing, but we cannot pool samples for you.
  • High Quality Library: libraries that contain a high level of adapter dimers will yield significantly less data, as the fragments will hybridize to the flowcell. Similarly, over amplified libraries can negatively impact yield.
  • Uniform Base composition: libraries that have uneven base composition tend to pose problems with the MiSeq analysis software. These issues can be mitigated using several strategies, but the net effect will be lower data yield per lane than a balanced library.

Please see Illumina’s MiSeq Specification page for current data yields.

Data Delivery

Data will be returned in Sanger FASTQ format via our high speed aspera server.

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