At the GRCF High Throughput Sequencing Center, our goal is to provide the research community at Johns Hopkins University with access to cutting edge sequencing platforms. To that end, we are offering Oxford Nanopore Sequencing on the GridION platform.
The Oxford Nanopore’s unique direct molecule sequencing platform is based on protein nanopores set in a polymer membrane. Current is passed through the nanopore, and as DNA/RNA is passed through the pore, a disruption in current is detected.
DNA Sequencing (from nanoporetech.com): A strand of DNA is passed through a nanopore. The current is changed as the bases G, A, T and C pass through the pore in different combinations
Oxford Nanopore can produce ultra-long read sequencing reads, limited by DNA length/quality. In our hands we regularly generate reads >100kb. There are a host of applications, including:
- Rapid sequence identification
- Utilizing long reads for improved genome assembly
- Analysis of full length RNA transcripts from cDNA (PCR and PCR-free)
- Direct sequencing of RNA molecules
- Metagenomic analysis
- Structural variant detection
- Copy Number detection in complex regions
Library prep: $120/sample DNA, $175/sample RNA
Sequencing: $500 per flowcell
Data yield: varies by application