At the GRCF High Throughput Sequencing Center, our goal is to provide the research community at Johns Hopkins University with access to ‘next generation’ sequencing platforms. We currently feature MiSeq and NovaSeq6000 instruments. We do our best to sequence samples in a timely manner, but our primary focus is on generating high quality data.
We offer simple, per lane and per flow cell pricing, along with end to end service for whole exome/custom targeted projects, RNASeq, and whole genome. We now offer single lane loading on NovaSeq SP to accommodate users who do not need the throughput of a full flowcell.
NovaSeq Flowcell Pricing
NovaSeq Flowcell Order
NovaSeq runs include additional cycles for dual indexed libraries.
If you need to individually load lanes on the NovaSeq, it adds $150/lane
| SP: ~1.6 billion paired reads, ~800 million single reads | S1: ~3.2 billion paired reads, ~1.6 billion single reads | S2: ~8.2 billion paired reads, ~4.1 billion single reads | S4: ~20 billion paired reads, ~10 billion single reads |
|---|---|---|---|
| 100 cycle: $3600 | 100 cycle: $6000 | 100 cycle: $9300 | |
| 200 cycle: $4300 | 200 cycle: $7000 | 200 cycle: $11100 | 200 cycle: $15000 |
| 300 cycle: $4600 | 300 cycle: $7500 | 300 cycle: $11600 | 300 cycle: $17400 |
| 500 cycle: $5800 |
NovaSeq Per Lane Pricing
NovaSeq Lane Order| SP: ~800 million paired reads, ~400 million single reads |
|---|
| 100 cycle: $1950 |
| 200 cycle: $2300 |
| 300 cycle: $2450 |
| 500 cycle: $3050 |
HiSeq2500 Flowcell Pricing (Rapid Mode)
We no longer offer HiSeq2500 runs due to cost. Please consider ordering a single lane on NovaSeq. If you have a legacy project that requires HiSeq, please contact David Mohr.
Sample Requirements
Completed Libraries: For NovaSeq runs, please submit at least 50ul of your sample at 4nM. Please submit 10μl of your sample at 2nM for MiSeq. Samples must be pooled. We will do a QC check via Bioanalyzer to increase the likelihood of quality data, but you should quantitate your sample as accurately as possible. Nanodrop is not reliable. Intercalating dye methods or qPCR are preferred.
Library Prep: $250/sample. Please provide us with ~500ng of high molecular weight DNA or total RNA >6.5 for this price. There are a host of options for lower input/lower quality that may add cost. Please contact us to discuss sample submission.
Data Yield
Our facility features the NovaSeq platform. Yield is dependent upon several factors:
- Read Length: the longer the read, the more data.
- Read Type: paired end reads yield twice the data as single read.
- Optimal Cluster density: it is imperative to accurately quantitate your library to ensure high data yield. We do our best to QC libraries before sequencing, but we cannot pool samples for you.
- High Quality Library: libraries that contain a high level of adapter dimers will yield significantly less data, particularly on the NovaSeq6000. Similarly, over amplified libraries can negatively impact yield.
- Uniform Base composition: while less of an issue than in the past, libraries that have uneven base composition tend to pose problems with the analysis software. These issues can be mitigated using several strategies, but the net effect will be lower data yield than a balanced library.
Please see Illumina’s NovaSeq6000 Specification page for current data yields. While we regularly achieve greater than ‘spec’ yields from the NovaSeq6000, it is best to be conservative when planning your experiments.
Data Delivery
Per lane/flowcell sequencing: Data will be returned in Sanger FASTQ format via our high speed aspera server
End to end services: alignment files, variant calls, and any intermediate files you wish via aspera server
Place Order