We offer free consultation. Please contact David Mohr to discuss your project in detail.

At the GRCF High Throughput Sequencing Center, our goal is to provide the research community at Johns Hopkins University with access to ‘next generation’ sequencing platforms. We currently feature MiSeq and NovaSeq6000 instruments.  We do our best to sequence samples in a timely manner, but our primary focus is on generating high quality data.

We offer simple, per lane and per flow cell pricing, along with end to end service for whole exome/custom targeted projects, RNASeq, and whole genome.

NovaSeq6000 Lane Loading

We now offer single lane loading on NovaSeq SP to accommodate users who do not need the throughput of a full flowcell.

NovaSeq Flowcell Pricing

NovaSeq Flowcell Order
NovaSeq runs include additional cycles for dual indexed libraries.

If you need to individually load lanes on the NovaSeq, it adds $150/lane

SP: ~1.6 billion paired reads, ~800 million single readsS1: ~3.2 billion paired reads, ~1.6 billion single readsS2: ~8.2 billion paired reads, ~4.1 billion single readsS4: ~20 billion paired reads, ~10 billion single reads
100 cycle: $3600 100 cycle: $6000 100 cycle: $9300
200 cycle: $4300200 cycle: $7000200 cycle: $11100200 cycle: $15000
300 cycle: $4600300 cycle: $7500300 cycle: $11600300 cycle: $17400
500 cycle: $5800

NovaSeq Per Lane Pricing

NovaSeq Lane Order
SP: ~800 million paired reads, ~400 million single reads
100 cycle: $1950
200 cycle: $2300
300 cycle: $2450
500 cycle: $3050

HiSeq2500 Flowcell Pricing (Rapid Mode)

We no longer offer HiSeq2500 runs due to cost. Please consider ordering a single lane on NovaSeq. If you have a legacy project that requires HiSeq, please contact David Mohr.

Sample Requirements

Completed Libraries: For NovaSeq runs, please submit at least 50ul of your sample at 4nM. Please submit 10μl of your sample at 2nM for MiSeq.  Samples must be pooled.  We will do a QC check via Bioanalyzer to increase the likelihood of quality data, but you should quantitate your sample as accurately as possible.  Nanodrop is not reliable.  Intercalating dye methods or qPCR are preferred.

Library Prep:  $250/sample.  Please provide us with ~500ng of high molecular weight DNA or total RNA >6.5 for this price.  There are a host of options for lower input/lower quality that may add cost.  Please contact us to discuss sample submission.

Data Yield

Our facility features the NovaSeq platform. Yield is dependent upon several factors:

  • Read Length: the longer the read, the more data.
  • Read Type: paired end reads yield twice the data as single read.
  • Optimal Cluster density: it is imperative to accurately quantitate your library to ensure high data yield. We do our best to QC libraries before sequencing, but we cannot pool samples for you.
  • High Quality Library: libraries that contain a high level of adapter dimers will yield significantly less data, particularly on the NovaSeq6000. Similarly, over amplified libraries can negatively impact yield.
  • Uniform Base composition: while less of an issue than in the past, libraries that have uneven base composition tend to pose problems with the analysis software. These issues can be mitigated using several strategies, but the net effect will be lower data yield than a balanced library.

Please see Illumina’s NovaSeq6000 Specification page for current data yields. While we regularly achieve greater than ‘spec’ yields from the NovaSeq6000, it is best to be conservative when planning your experiments.

Data Delivery

Per lane/flowcell sequencing:  Data will be returned in Sanger FASTQ format via our high speed aspera server

End to end services:  alignment files, variant calls, and any intermediate files you wish via aspera server

Place Order