Sanger Sequencing FAQs

  1. Why do I get an error when I try and access your ordering server? Genesifter access is now through URL http://orders.jhgenomics.jhu.edu and it is ONLY accessible within the Hopkins environment.  To access Genesifter from a remote location outside the JHMI domain firewall, log in to your JHED account (http://my.jh.edu) and follow the instructions in the sidebar VPN link. Once connected through Pulse Secure you will be able to access Genesifter using our new URL http://orders.jhgenomics.jhu.edu (remember to update Genesifter bookmarks).
  2. How should I provide my template and primer? Please provide your pre-mixed sample containing 6 µl of template and 6 µl of primer, at the following concentrations:
    1. Primer: 1 µM for PCR or Plasmid, 4 µM for BAC.
    2. PCR products: 3.0 ng/µl/100 bp.
    3. Plasmid DNA: 0.2 µg/µl.
    4. BAC/PAC DNA: 0.5 µg/µl.
  3. How much template should I provide if I want to use your primers? If you would like us to add one of our standard primers to your template, please bring 6µl of template in a tube and indicate the appropriate primer when you place your order. There is no charge for using our primers.
  4. How do I place a sequencing order? The JHU ordering server manages all orders and data. To place an order and view your data, you must have a username and password. If you do not have an account, you can set one up from the log-in page. Please use your PI’s name instead of the Institution to help us with the final step of the set up.
  5. Should each person in the lab have their own log-in?  In order to utilize all of the features of the server, including order notifications and data tracking, each individual in your lab should have their own login.  If your lab does not wish to do this, keep in mind that we will contact the person whose name is on the account with any questions or concerns.  This may cause a delay in processing your samples.
  6. When will my data be ready? Results for sequencing samples dropped off in Blalock by 4:00 PM will be available by 10:00 AM the following morning, assuming no machine problems. Samples dropped off in our remote locations by 11:00 AM will receive their data by 10:00 AM the following morning. You will receive an email when your data is completed.
  7. Where do I drop off my samples? 
    1. Blalock 10th floor: There are two drop off locations:
      1. Ross bridge side:  Once you cross the bridge, look for a small fridge immediately to your left when you go through the door.  The code for the lock should be in your order confirmation email.
      2. Blalock Elevator side:  There is a fridge behind the door in Blalock 1036.  Follow the signs to find room 1036.  The code for the lock should be in your order confirmation email.
    2. CRB I B02A (slot A63): Samples may be dropped off in slot A63.  Samples should be dropped off by 11 AM to be processed for data release the next day.
      1. First time users of the 24/7 system will need to email Lee Hilliard or call 410-502-3959 to set up and account. You may also download forms for 24/7 access. If you already have a 24/7 account, you can just login to the system and drop off the sample.
    3. Homewood:  Pickup will happen from the locations below after 12 noon on the days that the CoreStore makes deliveries to Homewood. As of 10/06/20, those days are Wednesday and Friday.
      1. UTL Rm 384 (Black refrigerator in the break room right across from the main staircase.)
      2. Croft Hall outside Suite 100 (Black refrigerator in the hallway. Please note this fridge is for DNA only.)
      3. Call 410-955-2836 for the combination to unlock the refrigerators.
      4. You may also hand your samples to the CoreStore personnel when they deliver to your lab.
    4. Bayview Campus: Drop off in the Core 24/7 on the first floor of the Asthma and Allergy building, in slot F63. Pickup will happen after 12 noon on the days that the CoreStore delivers to Bayview. As of 10/06/20, those days are Wednesday and Friday.  
      1. First time users will need to email Lee Hilliard or call 410-502-3959 to set up and account. You may also download forms for 24/7 access. If you already have a 24/7 account, you can just login to the system and drop off the sample. Someone is usually at the 24/7 from 11:00 AM to 12 PM to help with problems and questions.
  8. How should I submit my samples?
    1. Tubes: due to storage issues, we will accept only 1.5 ml eppendorf tubes. Plates: 24 samples or more may be submitted on plates with the requirement that all consecutive wells are filled in a top to bottom, left to right format (i.e. A1-H1; A2-H2, etc.).
    2. Labeling: Your tubes need to be labeled with your Genesifter Tracking ID and the position number.
    3. Note: Any sample that is improperly labeled, unreadable, or any sample sets organized in a way that we feel may increase the risk of error during handling may be rejected. Improperly labeled samples will be returned.
  9. Are there any tools I can use to view my data?
    1. The original developer of our ordering server created FinchTV as a free, basic tool for viewing data and doing limited assembly.  That developer still maintains the software even though they no longer own Genesifter.  Downloads are available. Other free and paid tools are available by searching the web.