Pyrosequencing

  1. Customers must supply a valid method of payment when ordering: I/O numbers for internal Hopkins customers, Credit Card or PO for external customers.
  2. Customers must supply either .xml file from the Qiagen design software (preferred) or our assay information files when placing an order.
  3. All orders require a gel picture.
  4. To be successful, the bands in the gel picture should be brighter than the equivalent band of the size standard.

Sanger Sequencing

  1. Customers must supply a valid method of payment when ordering: I/O numbers for internal Hopkins customers, Credit Card or PO for external customers.
  2. The customer is responsible for quantitating DNA and mixing with primer.  Remote drop off users may indicate that we should add our standard sequencing primers for them, but they should add their custom primers as needed.
  3. All samples that fail are rerun on the following business day.