Pyrosequencing

  1. Customers must supply a valid method of payment when ordering: I/O numbers for internal Hopkins customers, Credit Card or PO for external customers.
  2. Customers must supply either .xml file from the Qiagen design software (preferred) or our assay information files when placing an order.
  3. All orders require a gel picture.
  4. To be successful, the bands in the gel picture should be brighter than the equivalent band of the size standard.

Sanger Sequencing

  1. Customers must supply a valid method of payment when ordering: I/O numbers for internal Hopkins customers, Credit Card or PO for external customers.
  2. The customer is responsible for quantitating DNA and mixing with primer.  Custom primers should be premixed with the sample prior to being dropped off.  If you are using a standard sequencing primer, please indicate the appropriate primer on the order form and we will add the primer when we receive your samples.
  3. Failing samples will be rerun on the following business day.