The GRCF offers total RNA isolation from PAXgene blood collection tubes, tissues (fresh or RNAlater preserved), cultured cells and plasma samples. RNA is suitable for many downstream applications including cDNA synthesis, qPCR, Northern blots and library construction. The GRCF extracts RNA using Qiagen RNeasy Kits (RNeasy Plus and miRNeasy).
Quantitative analysis is by Nanodrop.
Qualitative analysis is available by Advanced Analytical Fragment Analyzer CE analysis. Advanced Analytical’s PROSize software determines a RQN (RNA Quality Number) on a scale from 1 to 10. Additional fees apply at $14 per sample. Discounts for full runs (10 samples) or full plates (92 samples).
Please contact Laura Kasch [410-614-3830] for additional information.
(without CE analysis)
|Blood||PAXgene collection tube||$30|
|Tissue_Total RNA||< 30mg||$32|
|Tissue_Total RNA||< 5mg||$32|
|Tissue_Total RNA plus miRNA and small RNA||< 30mg||$40|
|Cultured Cells_Total RNA plus miRNA and small RNA||< 1 x 10E7||$35|
|Cultured Cells_Total RNA||< 1 x 10E7||$27|
|Cultured Cells_Total RNA||<5 x 10E5 cells||$27|