cDNA Synthesis for MicroRNA Profiling and Custom Gene Expression Taqman Arrays
The GRCF offers cDNA synthesis to support customers requesting Taqman OpenArray MicroRNA Profiling Analysis or custom and fixed content Gene Expression Array analysis on the QuantStudio 12K Flex Real-Time PCR System. cDNA synthesis is carried out following recommended protocols using customer supplied total RNA or RNA isolated by GRCF DNA Services for start to finish processing.
- RNA should be free of PCR inhibitors
- Has a A260/230 ratio between 2.0 and 2.4
- Has a A260/280 ratio between 1.8 and 2.1
- Has a RNA Integrity Number (RIN) or RNA Quality Number (RQN) between 6.5 and 10
The recommended amount of total RNA for preparing cDNA for microRNA Profiling is 100ng.
The recommended amount of total RNA for preparing cDNA for Gene Expression Taqman Arrays is 10µl at 200ng/µl (2µg).
Qualitative analysis of RNA samples is available by Advanced Analytical Fragment Analyzer CE analysis. The system determines a RQN (RNA Quality Number) of total RNA on a scale from 1 to 10 that correlates to current industry practices (RIN).
cDNA synthesis for custom and fixed content Gene Expression Array analysis using Superscript VILO (Life Technologies part # 4453650: $25 per sample
cDNA synthesis for MicroRNA profiling using High Capacity cDNA Reverse Transcription Kit (Life Technologies part # 4368813): $27 per sample for A and B pools
Please contact Laura Kasch [410-614-3830] for ordering.