Analysis via Pyrosequencing
Specific regions of methylation can be assayed via sequencing on the Pyromark Q48 system from Qiagen, using their advanced methylation reagents. This is not high throughput/next generation sequencing. Bisulfite converted DNA is PCR amplified with a biotinylated primer pair. The non-biotinylated strand is sequenced in the Pyromark system. A video describing the process is available on YouTube.
Custom regions can be amplified, or one of the 30,000 human and rodent predesigned kits can be ordered from Qiagen. Primers ordered through us receive a slight discount and free shipping. Assay design assistance is available for all projects being run in our facility.
For more information, contact Roxann Ashworth [410-614-0702]
run at one time with the same assay
PCR and Bisulfite Conversion Support Service
Full Service: includes bisulfite conversion of DNA samples (if needed), PCR of samples, amplification confirmation of the PCR by agarose gel electrophoresis and submission for Pyrosequencing. Please contact Laura Kasch [410-614-3830] for more information.
PCR Only Support: PCR support pricing is sample number dependent. Please inquire.