Bisulfite conversion of isolated DNA is offered using Zymo Research Corporation EZ DNA Methylation Kit or Qiagen EpiTect Bisulfite Kits. Converted DNA is suitable for PCR, endonuclease digestion, sequencing, microarrays, and Illumina Infinium and Golden Gate Methylation Assays.
Illumina Methylation Studies
Illumina recommends the following kits for bisulfite conversion.
- EZ DNA Methylation Kit for 50 DNA reactions (Zymo Research, catalog # D5001)
- EZ DNA Methylation Kit for 200 DNA reactions (Zymo Research, catalog # D5002)
- EZ-96 DNA Methylation Kit for 2×96 DNA conversion reactions (deep-well Zymo-Spin I-96 Filter Plate) (Zymo Research, catalog # D5004)
Either type of bisulfite conversion kit may be used for the Pyromark system. Qiagen guarantees their Pyrosequencing assays if the EpiTect bisulfite conversion kit is used.