Bisulfite conversion of isolated DNA is offered using Zymo Research Corporation EZ DNA Methylation Kit or Qiagen EpiTect Bisulfite Kits. Converted DNA is suitable for PCR, endonuclease digestion, sequencing, microarrays, and Illumina Infinium and Golden Gate Methylation Assays.
Illumina Methylation Studies
Illumina recommends the following kits for bisulfite conversion. They do not recommend using any other kits.
- EZ DNA Methylation Kit for 50 DNA reactions (Zymo Research, catalog # D5001)
- EZ DNA Methylation Kit for 200 DNA reactions (Zymo Research, catalog # D5002)
- EZ-96 DNA Methylation Kit for 2×96 DNA conversion reactions (deep-well Zymo-Spin I-96 Filter Plate) (Zymo Research, catalog # D5004)
Either type of bisulfite conversion kit may be used for the Pyromark system, though the assays are guaranteed by Qiagen if their conversion kit is used.
EpiTect Bisulfite conversion: $15/sample. EZ DNA Methylation conversion pricing is sample number dependent. Please contact Laura Kasch for details, pricing and ordering.