How many cells will I need to start my experiment?
The number of cells needed to start your experiment is directly dependent on the target number of cells for capture in the 10x Genomics Chromium platform. In Fluidigm’s C1 Single Cell Auto Prep system, we ask for minimum of 8-10,000 cells. In both systems it is essential to assess the quality and viability of the cells prior to loading for cell capture. In planning your experiment please plan to provide more cells than will be needed for your run.
What do I need to bring to the GRCF Biorepository & Cell Center to start my experiment?
Everyday the GRCF is expanding on the single cell isolation capabilities. To date we have experience with isolation of cells from primary tissues, primary cell isolates, stem cell cultures and cell lines. Prior to starting a single cell genomics experiment a consultation is required to better understand the scope and needs of your project.
Will my single cell RNAseq data be analyzed when I receive it?
The 10x Genomics Chromium platform offers a Linux- based pipeline that converts raw sequencer output to filtered single cell expression matrices and supporting graphs. For experiments completed with the 10x Genomics Chromium system, the GRCF will provide both raw and pipeline analyzed data. Data generated from the Fluidigm C1 system will not be analyzed by the GRCF. In both cases, it’s important to establish how the data will be analyzed before the experiment begins.