The GRCF is excited to now offer a growing range of genomics applications for single cells, including RNA-seq, gene expression profiling by qPCR and DNA amplification for whole-genome or targeted (exome or PCR-based analysis) through 10x  Genomics Chromium platform (similar to Drop Seq).


Drop Seq technology as presented through the 10x Genomics Chromium Platform 10x genomics chromium pictureoffers a high throughput molecular barcoding and analysis suite that delivers cell-by-cell 3’end counting of mRNA transcripts for many tens of thousands of cells per run- similar to the popular DropSeq technology. This technology supports a broad range of applications, including cancer-cell transcriptomics and cell-type identification and discovery.  Because the platform works with short read sequencers, it integrates easily into the existing GRCF RNAseq workflow allowing for an end to end service.

 

10x workflow

Click Here for a 10x Genomic Single Cell Overview

 

 

The well-characterized pipeline and unique software, allows the GRCF to offer analysis for 10X Genomics data.  The GRCF cost for sequencing will vary based on read length.

Single cell genomics through the GRCF is a  joint effort between the GRCF Biorepository & Cell Center and GRCF DNA services center to allow for a one-stop single cell isolation, sequencing and analysis service.

Contact us today for more information or to schedule for your experiment!

410-614-5201

bioprocessing@jhmi.edu