The GRCF Biorepository & Cell Center is able to help you with your cell culture needs. We are a mycoplasma-free facility offering primary fibroblast establishment, cell culture expansion and cell line distribution.

Cell Expansion / Preparation of Cell Line Stocks:

Cultured cells from your lab can be tested for mycoplasma, expanded, aliquoted, and cryopreserved in freezing media (5 x 106/vial).

Preparation of cells for DNA or RNA extraction:

Eukaryotic cells from the your lab or from ATCC can be expanded for nucleic acid isolation (1 x 10 8 cells). DNA or RNA extraction is available through the Fragment Analysis Facility.

Establishment of fibroblast cultures from Tissue Biopsies:

Service includes fibroblast establishment in culture, cryopreservation of four (4) aliquots (5 x 106 cells per vial) and electronic sample tracking. Submit 5 X 2mm biopsy and allow 8-10 weeks for completion.

Establishment of cell lines from frozen lymphocytes:

Service includes B-cell transformation from previously cryopreserved blood lymphocytes, culture expansion, and cryopreservation. Specifically includes: Thaw of cryopreserved lymphocytes, cell count, viability determination, 1-4 aliquots of freshly cryopreserved lymphocytes @ 5 x 106 cells, and 4 aliquots of (transformed) lymphoblasts @ 5 x 106 cells and electronic sample tracking. Allow 8-10 weeks for completion.

Cell Culture Maintenance for Specific Projects:

Service includes expanding cells from your lab or ATCC according to your long-term project need and providing you with live cell flasks for your experiment. throughout your project. Cells will also be tested for mycoplasma and cryopreserved @ 5 X 106 cells /vial as your back up.

Cell Line Distribution:

Service includes cryopreserved cell line stock and maintenance (5 x 106 cells per vial), arrangements, and shipment (domestic or international) to collaborators of your choosing.

We are committed to assisting you with any cell culture questions such as contamination, slow-growing cell lines, techniques, lab design, and lab maintenance. Please feel free to contact, Melissa Olson, Ph.D. or Kakali Sarkar, Ph.D. (ksarkar4@jhmi.edu) to schedule a time to discuss your concerns.