Vac2B

 GRCF Biorepository & Cell Center can help you with your blood processing needs. We offer blood isolation, aliquoting and / or cryopreservation of whole blood, lymphocytes, sera and plasma as well as LCL establishment. 

  Instructions for Blood Collected Internally at JHUInstructions for Blood Collected Outside of JHU

Isolation and cryopreservation of viable lymphocytes from whole blood:

Service includes sterile lymphocyte isolation by ficoll gradient, cell count, viability determination,

cryopreservation of 1 ml aliquot whole blood, cryopreservation of 1-4 aliquots of isolated lymphocytes @ 5 x 106 cells, and electronic sample tracking. Cryopreserved lymphocytes are suitable for future transformation. Same-day service available if whole blood is received by 2pm.

 

Establishment of cell lines from isolated lymphocytes or whole blood (LCLs):

Service includes B-cell transformation from whole blood isolated blood lymphocytes, culture expansion, and cryopreservation. Specifically includes: lymphocyte isolation by ficoll separation, cell count, viability determination, cryopreservation of whole blood aliquot (1 ml), 1-4 aliquots of lymphocytes @ 5 x 106 cells, and 4 aliquots of (transformed) lymphoblasts @ 5 x 106 cells and electronic sample tracking. Allow 8-10 weeks for completion.

 

Establishment of cell lines from frozen lymphocytes:

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Service includes B-cell transformation from previously cryopreserved blood lymphocytes, culture expansion, and cryopreservation. Specifically includes: Thaw of cryopreserved lymphocytes, cell count, viability determination, 1-4 aliquots of freshly cryopreserved lymphocytes @ 5 x 106 cells, and 4 aliquots of (transformed) lymphoblasts @ 5 x 106 cells and electro

nic sample tracking. Allow 8-10 weeks for completion.

 

Isolation, aliquoting, and cryopreservation of sera and plasma:

Service includes isolation and cryopreservation of sera and plasma aliquots (0.5-1ml) with electronic sample tracking. Cryopreserved sera and plasma proteins are intact for future analysis. Same-day service available if whole blood is received by 2pm.

Aliquoting and cryopreservation of whole blood:

Service includes the aliquoting fresh whole blood in 0.5-1ml volumes and cryopreserving.

 

Cell Pellet for DNA Isolation

 

** Please Note ** For your convenience the GRCF Biorepository & Cell Center now has after hours drop boxes for blood processing!  Blood deposited in one of these three locked boxes, will be processed at 7:30am the following day.

 

To discuss blood processing needs not mentioned here, please contact Melissa Olson, the center Director (mvolson@jhu.edu).