GRCF

The Genetic Resources Core Facility (GRCF) is a Johns Hopkins Universityservice center with six divisions. Collectively, these divisions produce a number of products and services to aid researchers performing studies in molecular biology and genetics. It is our mission to provide solutions to the everyday challenges of laboratory research. Additionally, the GRCF sponsors various educational programs, product seminars and an annual symposium.

We strive to provide high quality, economical products and services to the Hopkins community and we look forward to your advice and input on how we can improve our services. Feel free to drop us a line

Core Store

The CORE store has a 19 year history of serving the Hopkins research community by providing an economical, reliable source of reagents for the research laboratory. In addition to over 685 stocked products from 20 of the leading life science suppliers, special orders can also be placed with many of these companies. For a complete listing of all 83,000 products available through the store go to our ordering website at jhucorstore.com

The store provides the following services:

  • Convenient on-line ordering
  • 24/7 access at 1026 Blalock and the CRB II
  • Delivery service to East Baltimore, Bayview and Homewood campuses
  • Free shipping and handling
  • Discounted products
Core Store Homepage

Cell Center

The Cell Center was established in 1989 to facilitate basic scientific research by providing expertise and service in all aspects of mammalian cell culture. Our primary service is establishing immortalized cell lines from mononuclear cells isolated from study participants' peripheral blood (EBV transformations). Other services include:

  • The establishment and/or growth of other types of cells
  • Repository services (banking and shipping samples)
  • Monoclonal antibody production/purification
  • Mycoplasma testing
  • Consulting/training in cell culture

The Cell Center is a member of ISBER (the International Society for Biological and Environmental Repositories) and adheres to ISBER's Best Practices for Repositories.

cellcenter.grcf.jhmi.edu

SNP Center

The JHU SNP Center is a high through-put genotyping facility featuring Illumina's BeadArrayTM technology, serving JHU and the scientific community at large. This technology allows for the analysis of thousands of genotypes on a relatively small amount of DNA.

We feature a wide variety of fixed content products, including Genome Wide Association (GWA) chips, Linkage products, and specialty products such as non-synonomous SNP panels and methylation panels. We also assist customers with the creation of custom SNP panels.

Be sure to check out the Pricing section of our site for our new, lower pricing on GWA products.

snpcenter.grcf.jhmi.edu

DNA Analysis Facility

The DNA Analysis Facility provides automated fluorescent sequencing and oligonucleotides to the Hopkins Community. We strive to provide our customers with high quality data at an affordable cost. Using the JHU Finch Server, we are able to capture orders, to distribute data, and to store it indefinitely. Data produced using the Applied Biosystems 3730xl DNA Analyzer is then made available for download, for online or offline viewing, and for editing through the conveniences of the web-based JHU Finch Server. Tracking your data has never been easier. Please view our How-to section for more information on using the power of the JHU Finch Server.

We now offer oligonucleotides through Sigma-Genosys, allowing you to enjoy the convenience of ordering through the DNA Analysis Facility coupled with the low pricing of large manufacturing. Orders are placed through the JHU Finch Server and delivered with your regular Core Store deliveries, if you desire. Bulk ordering, sample tracking, indefinite storage, and integration with DNA sequencing are just few of the benefits of using the JHU Finch Server. daf.jhmi.edu

Fragment Analysis Facility

The Fragment Analysis Facility was established in 1994 to facilitate scientific research by providing technical assistance with low throughput genotyping and genomic DNA isolation.

The FAF offers genomic DNA isolation from whole blood, buffy coats, packed cells, cultured cells, blood spot cards, buccal swabs, mouthwash collection, Oragene saliva collection kits, and FFPE samples. FAF genotyping services include SNP analysis by Taqman, fluorescent fragment analysis for determination of STR or VNTR number, analysis of insertion/deletions, and SNP discovery by sequencing. Whole genome amplification, DNA archiving, and sample dispensing and shipping are also available.

faf.jhmi.edu

High Throughput Sequencing Facility

The GRCF HTS Center was established to provide the research community at Johns Hopkins University with access to 'next generation' sequencing platforms. We currently feature an Illumina Genome Analyzer II, which has the capability to generate sequences with high accuracy at an extremely low cost per base. Using a sequencing-by-synthesis chemistry, billions of bases are produced with each run at a cost of less than 1% of capillary based methods.

The GAII offers some exciting capabilities. Among them are resequencing, de novo sequencing, SNP discovery, transcript counting, small RNA sequencing, ChIP-seq, and DNA Methylation sequencing.

Both single read and paired-end sequencing with read lengths of up to 75 bp are offered.

grcf.jhmi.edu/hts

Services

This is a list of services available within the Genetic Resources Core Facility
DNA Isolation and Storage Genotyping Live Cells Sequencing Shipping Special Analysis

Locations

GRCF Administrative Offices: Blalock 1031-1035
Cell Center: Blalock 1017 Ph 410-614-5201
Core Store: Blalock 1026 Ph 410-955-3321
Core Store 24/7:Blalock 1026CRB II LB-06, Asthma and Allergy Center 1st Floor
DNA Analysis Facility: Blalock 1005 Ph 410-955-2836
Additional Drop Off Locations: CRB II Room LB-06 and Asthma and Allergy Center 1st Floor
Fragment Analysis Facility: 2760 Lighthouse Point East, Suite 201,Baltimore MD 21224 Ph 410-614-3830
Additional Sample Drop Off Locations: CRB II, Room LB-06
High Throughput Sequencing: 2760 Lighthouse Point East, Suite 200, Baltimore, MD 21224 Ph 410-614-3648
SNP Center: 1004 Blalock Ph 410-859-3085

Grant Resources

Writing a grant and need justification for utilizing the GRCF? Here are the answers you need. Below you will find Resource and Environment (R&E) documents for various division of the GRCF.

Events

  • 9/10/09: Core Store Supplier Exhibit - Life Science suppliers will be present to exhibit the latest promotions and products available through the Core Store. This will take place in the BRB Pre-function area on the Turner Concourse from 11:00 a.m. - 1:00 p.m.